Rabbit Monoclonal Antibodies | St John's Laboratory

Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit mAb (STJ11102639) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-SMC1A-S957 antibody [S9MR] (STJ11102639)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-SQSTM1/p62-Ser349 antibody [PT0993R] (STJA0040063)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-Src Family-Tyr416 antibody [PT0664R] (STJA0039167)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Western blot analysis of lysates from C6 cells using Phospho-SRC Family-Y416 Rabbit monoclonal antibody (STJ11105850) at 1:1000 dilution. C6 cells were treated by Pervanadate (0. 5 mM) at 37 °C for 40 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-SRC Family-Y416 antibody [S5850RM] (STJ11105850)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (STJ11102608) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SRC-Y419 antibody [S8MR] (STJ11102608)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of A-431, using Phospho-Src-Y529 antibody (STJ11105854) at 1:10000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-SRC-Y529 antibody [S5854RM] (STJ11105854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates using Phospho-STAT1-S727 Rabbit mAb (STJ11102589) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-STAT1-S727 antibody [S9MR] (STJ11102589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-STAT1-Ser727 antibody [PT0994R] (STJA0040252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-STAT1-Tyr701 antibody [PT0997R] (STJA0040064)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Western blot analysis of various lysates using Phospho-STAT1-Y701 Rabbit mAb (STJ11102552) at 1:1000 dilution incubated overnight at 4 °C. HeLa, NIH/3T3 and PC-12 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-STAT1-Y701 antibody [S2MR] (STJ11102552)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-Phospho-STAT2-Y690-Y690 antibody [SRM15145] (STJ11109875)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

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Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (STJ11102565) at 1:1000 dilution. NIH/3T3 and PC-12 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-STAT3-S727 antibody [S5MR] (STJ11102565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-STAT3-Ser727 antibody [PT0911R] (STJA0039802)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-STAT3-Tyr705 antibody [PT0742R] (STJA0039211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (STJ11102563) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-STAT3-Y705 antibody [S3MR] (STJ11102563)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-STAT3-Y705 Rabbit monoclonal antibody (STJ11105819) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-STAT3-Y705 antibody [S5819RM] (STJ11105819)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-Phospho-STAT3-Y705-Y705 antibody [SRM15135] (STJ11109865)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-STAT5-Tyr694 antibody [PT0067R] (STJA0039276)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-STAT5-Y694 antibody [SRM13712] (STJ11108442)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-STAT5-Y694-Y694 antibody [SRM15149] (STJ11109879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-STAT5A-Y694 antibody [SRM13682] (STJ11108412)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-STAT6-Tyr641 antibody [PT0917R] (STJA0039807)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-STING/TMEM173-S365-S365 antibody [SRM15156] (STJ11109886)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£0.00

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Western blot analysis of THP-1, using Phospho-STING/TMEM173-S366 antibody (STJ11105849) at 1:1000 dilution. THP-1 cells were transfection with poly (dA:dT) (5 Mu g/mL) for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-STING1-S366 antibody [S5849RM] (STJ11105849)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of C6, using Phospho-Stathmin 1-S25 Rabbit monoclonal antibody (STJ11105891) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-STMN1-S25 antibody [S5891RM] (STJ11105891)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Anti-Phospho-Syk-Tyr525/526 antibody [PT0753R] (STJA0039170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-Phospho-Synuclein-Alpha-Ser129 antibody [PT1002R] (STJA0040308)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various lysates using (STJ11103659) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-TAK1-S439 antibody [S9MR] (STJ11103659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Anti-Phospho-Tau-S214 antibody [SRM13692] (STJ11108422)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-Tau-Ser396 antibody [PT0897R] (STJA0039790)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-Tau-T181-T181 antibody [SRM15126] (STJ11109856)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-Tau-T205 antibody [SRM13695] (STJ11108425)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Mouse/Rat

£0.00

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Western blot analysis of recombinant Human p-Tau217 Protein using Phospho-Tau-T217 Rabbit mAb (STJ11105851) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 100ng Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 5s.

Anti-Phospho-Tau-T217 antibody [S5851RM] (STJ11105851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£143.50 - £618.50

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Anti-Phospho-Tau-T217-T217 antibody [SRM15124] (STJ11109854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of various lysates, using Phospho-TBK1/NAK-S172 Rabbit monoclonal antibody (STJ11105874) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-TBK1-S172 antibody [S5874RM] (STJ11105874)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£143.50 - £618.50

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Western blot analysis of various lysates using Phospho-TBK1/NAK-S172 Rabbit mAb (STJ11102607) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-TBK1-S172 antibody [S7MR] (STJ11102607)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Phospho-TBK1-Ser172 antibody [PT0905R] (STJA0039797)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from NIH 3T3 or NIH 3T3 treat with Calyculin A, using Pan Phospho-Threonine Rabbit monoclonal antibody (STJ11105878) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-Threonine antibody [S5878RM] (STJ11105878)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Anti-Phospho-TIFA-T9 antibody [SRM13697] (STJ11108427)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-TIFA-T9-T9 antibody [SRM15132] (STJ11109862)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-TMEM173-Ser366 antibody [PT1380R] (STJA0040278)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Mouse

£150.00 - £540.00

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Immunohistochemistry analysis of paraffin-embedded Human heart using Phospho-Cardiac Troponin I (TNNI3) -S23/S24 Rabbit monoclonal antibody (STJ11105867) at dilution of 1:700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-TNNI3-S23/S24 antibody [S5867RM] (STJ11105867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit mAb (STJ11102575) at 1:1000 dilution. HT-29 cells were treated by nocodazole (100 ng/mL) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-TP53-S33 antibody [S5MR] (STJ11102575)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from 293T cells, using Phospho-p53-S376 Rabbit mAb (STJ11102578) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-TP53-S376 antibody [S8MR] (STJ11102578)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of lysates from C6 cells, using Phospho-p53-S392 (STJ11102634) at 1:1000 dilution. C6 cells were treated by Paclitaxel (100 nM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-TP53-S392 antibody [S4MR] (STJ11102634)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S9 Rabbit mAb (STJ11102576) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-TP53-S9 antibody [S6MR] (STJ11102576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western blot analysis of lysates from 293T cells, using Phospho-p53-T55 Rabbit mAb (STJ11102577) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-TP53-T55 antibody [S7MR] (STJ11102577)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Trim24-S1043 Rabbit mAb (STJ11105806) at1:1000 dilution. Hela cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-Trim24-S1043 antibody [S5806RM] (STJ11105806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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