Loading Control Antibody | St John's Laboratory

Western blot analysis of various lysates using LACTB Rabbit polyclonal antibody (STJ11102333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LACTB antibody (120-290) (STJ11102333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-LACTB antibody (STJA0007134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LACTB2 antibody (19-69 aa) (STJ195523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ72853 (1µg/ml) staining of HeLa (A) and Jurkat (B) nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Lamin B1 antibody (526-537) (STJ72853)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Dog/Pig/Cow

£312.50

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STJ72852 (0. 2µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Lamin B1-Isoform 1 antibody (Internal) (STJ72852)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Pig/Cow

£312.50

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Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (STJ24413) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LMNB1 antibody (397-588) (STJ24413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (STJ119228) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-LMNB1 antibody (503-588) (STJ119228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Lamin B1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-LMNB1 antibody (527-540 aa) [R05-8D6] (STJA0010488)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£679.50

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Anti-Pan-Alpha Tubulin antibody [SM5020] (STJA0005020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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TRAF6 regulates autophagy and apoptosis of melanoma cells through c-Jun/ATG16L2 signaling pathway. Cong Peng IHC mouse 1:350 SK-Mel-5 cell-xenograft

Anti-PCNA antibody (1-100 aa) [ABT221] (STJ197033)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various lysates using PCNA Mouse mAb (STJ11107550) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-PCNA antibody (1-261 aa) [SMM] (STJ11107550)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using PCNA Mouse mAb (STJ11107563) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.

Anti-PCNA antibody (1-261 aa) [SMM] (STJ11107563)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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STJ72849 (0. 05µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PCNA antibody (111-122) (STJ72849)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Immunohistochemistry analysis of paraffin-embedded rat testis using PCNA Rabbit polyclonal antibody (STJ115299) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PCNA antibody (162-261) (STJ115299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded rat testis using PCNA Rabbit polyclonal antibody (STJ24918) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PCNA antibody (162-261) (STJ24918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PCNA antibody (162-261) [S3MR] (STJ11101413)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/African green monkey

£146.50 - £642.50

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Western blot analysis of lysates from 1) HepG2, 2) HuvEC, 3) COS7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-PCNA antibody (61-110 aa) (STJ94982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Rabbit

£48.00 - £252.00

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Anti-PCNA antibody (C-Term) (STJ13101593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of follicular center cells

Anti-PCNA antibody (Full length) [ZR378] (STJ180587)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £533.50

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STJ72848 (0. 01µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PCNA antibody (Internal) (STJ72848)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Western blot analysis of Hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour) cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PCNA antibody (Internal) (STJ98857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, 14-3-3 Theta/Tau (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-PCNA antibody [12D10] (STJ96933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-PCNA antibody [12D10] {AbFluor™ 350} (STJA0006137)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {AbFluor™ 405} (STJA0006138)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {AbFluor™ 488} (STJA0006139)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {AbFluor™ 555} (STJA0006140)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {AbFluor™ 594} (STJA0006141)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {AbFluor™ 647} (STJA0006142)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {AbFluor™ 680} (STJA0006143)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {Biotin} (STJA0006144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {Cy3} (STJA0006145)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {Cy5} (STJA0006146)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {FITC} (STJA0006147)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-PCNA antibody [12D10] {HRP} (STJA0006148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Western blot detection of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

Anti-PCNA antibody [2E1-G10-H10] (STJ99203)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC/IHC

Reactivity :

Human/Mouse/Monkey/Rat/Hamster

£144.00 - £348.00

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Western blot analysis of PCNA. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-PCNA antibody [SM4907] (STJA0004907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ICC

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of various lysates using PCNA Mouse mAb (STJ11106985) at 1:1000 dilution. <br> Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 45s.

Anti-PCNA antibody [SMM] (STJ11106985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Biotinylated STJ72849 (0. 05ug/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-PCNA, Biotinylated antibody (111-122) {Biotin} (STJ73513)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Biotinylated STJ72848 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-PCNA, Biotinylated antibody (Internal) {Biotin} (STJ73207)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Anti-Phospho-ACTB-Tyr-53 antibody (STJA0005242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TPPP-Ser18 antibody (STJA0009966)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-TUBA1C-Ser-165 antibody (STJA0005627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-TUBB3-Ser-172 antibody (STJA0005631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-VCL-Tyr821 antibody (786-835 aa) (STJ91311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Recombinant-alpha Tubulin antibody [SM0725] {HRP} (STJA0010725)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-Recombinant-alpha Tubulin antibody [SM5069] (STJA0005069)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ICC

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of Alpha-Tubulin at dilution of 1:2000

Anti-Recombinant-alpha Tubulin antibody [SR0621] (STJA0010621)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ICC

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-Recombinant-beta III Tubulin antibody [SM0723] {HRP} (STJA0010723)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Loading Control Antibodies