Anti-beta Tubulin antibody (C-Term) (STJ13100428)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the c-terminal region of human Tubulin beta chain conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus.
General Information
| Short Description | Rabbit polyclonal anti-beta Tubulin (C-Term) for use in IHC and WB in Mouse, Rat, Human, Xenopus and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat/Human/Xenopus/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1: 100 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | TUBB |
| Gene ID | 203068 |
| Uniprot ID | TBB5_HUMAN |
| Immunogen | A synthetic peptide from the c-terminal region of human Tubulin beta chain conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus. |
| Immunogen Region | C-Term |
| Specificity | Specific for Tubulin beta chain. |
Additional Info
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Protein Name | Tubulin Beta ChainTubulin Beta-5 Chain |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-6798695Reactome: R-HSA-8854518Reactome: R-HSA-9679191 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Tubulin Beta Chain antibodyAnti-Tubulin Beta-5 Chain antibodyAnti-TUBB antibodyAnti-TUBB5 antibodyAnti-OK antibodyAnti-SW-cl.56 antibody |
Information sourced from Uniprot.org