Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from Rat heart, using AFM Rabbit polyclonal antibody (STJ117448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.

Anti-AFM antibody (260-599) (STJ117448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£110.50 - £505.50

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Anti-AFM antibody (STJ192180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-AFMID antibody (STJA0007499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Alpha-Fetoprotein (AFP) antibody (STJ11104685) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (19-609) [S4685RM] (STJ11104685)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of extracts of HeLa cells, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) mouse monoclonal antibody (STJ119899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (19-609) [S9MM] (STJ119899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-AFP (PT0679) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HepG2 Lane 3: mouse liverPredicted band size: 69kDaObserved band size: 69kDa

Anti-AFP antibody (350-450 aa) (STJ196165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of various lysates, using Alpha-Fetoprotein (AFP) Rabbit polyclonal antibody (STJ22541) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (360-609) (STJ22541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of mouse liver, mouse serum cells using AFP Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-AFP antibody (371-420 aa) (STJ96505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) Rabbit polyclonal antibody (red, A17457) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AFP antibody (450-550) (STJ113691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot with Anti-AFP Antibody (STJ500051) and peptides and human serum albumin at 1:500 dilution in buffer.

Anti-AFP antibody (Full Length) (STJ500051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£400.50

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Anti-AFP antibody (Full Length) {Biotin} (STJ500052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£497.50

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Anti-AFP antibody (Full Length) {FITC} (STJ500053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£497.50

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, AFP alpha 1 Fetoprotein monoclonal antibody (17C5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-AFP antibody [17C5] (STJ96977)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£49.00 - £261.00

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Anti-AFP antibody [PT0713R] (STJA0039180)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-AFP antibody [R06-3X-1] (STJA0033731)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£214.50 - £308.50

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Alpha-Fetoprotein (AFP) Mouse monoclonal antibody (STJ113435) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AFP antibody [S5MM] (STJ113435)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-AFP/Alpha-fetoprotein antibody (Leu260-Val609) [1A179] (STJA0021998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£197.50 - £275.50

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Anti-AFP/Alpha-fetoprotein antibody [AF5] (STJA0019248)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ICC/IF/WB

Reactivity :

Human

£198.50 - £1,170.50

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Anti-AGA antibody (STJA0007857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AGA10 antibody (175-225 aa) (STJ196556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-AGAP2 antibody (STJA0009599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AGAP4 antibody (296-346 aa) (STJ195456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-AGAP5 antibody (417-467 aa) (STJ197598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AGAP8 antibody (339-389 aa) (STJ195592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AGAP9 antibody (4-54 aa) (STJ196083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AGBL1 antibody (723-773 aa) (STJ194623)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using CCP2 Polyclonal Antibody diluted at 1：2000

Anti-AGBL2 antibody (731-780 aa) (STJ92074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using AGBL4 Polyclonal Antibody

Anti-AGBL4 antibody (431-480 aa) (STJ91506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-AGBL5 antibody (499-549 aa) (STJ194382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-AGE antibody (STJ500054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey/Panda

£400.50

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Anti-AGE antibody {Biotin} (STJ500055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey/Panda

£497.50

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Anti-AGE antibody {FITC} (STJ500056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey/Panda

£497.50

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Western blot analysis of 3T3 cells using RAGE Polyclonal Antibody diluted at 1：1000

Anti-AGER antibody (133-182 aa) (STJ95358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Mouse lung using AGER Rabbit mAb (STJ11107310) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-AGER antibody (24-344 aa) [SRM5399] (STJ11107310)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Western blot analysis of lysates from Rat lung using Human AGER Rabbit polyclonal antibody (STJ11104862) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AGER antibody (24-344) (STJ11104862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using AGER antibody (STJ22543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AGER antibody (30-330) (STJ22543)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Rat lung, using AGER Rabbit polyclonal antibody (STJ115229) at 1:5000/1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AGER antibody (40-210) (STJ115229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AGER antibody [SRM14636] (STJ11109366)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-AGER antibody [SRM14637] (STJ11109367)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of extracts of various cell lines, using AGFG1 antibody (STJ115461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-AGFG1 antibody (1-280) (STJ115461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using AGFG1 antibody (STJ28216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AGFG1 antibody (1-280) (STJ28216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-AGFG1 antibody (STJ191953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from MCF-7 cells, using AGFG2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-AGFG2 antibody (91-140 aa) (STJ95316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-AGGF1 antibody (129-179) (STJA0006565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Aggrecan antibody (1440-1490 aa) (STJ13100710)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-Aggrecan antibody (20-70 aa) (STJ13100709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-Aggrecan antibody (200-250 aa) (STJ13100037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-Aggrecan antibody [70] (STJ16101898)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/IP/WB

Reactivity :

Human/Bovine

£775.50

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