Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates using CCKAR Rabbit polyclonal antibody (STJ11101179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CCKAR antibody (100-200) (STJ11101179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CCKAR antibody (215-264 aa) (STJ92072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using CCK-BR Polyclonal Antibody

Anti-CCKBR antibody (11-60 aa) (STJ92073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of lysates from human stomach cancer tissue, using CCKBR Rabbit polyclonal antibody (STJ22926). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CCKBR antibody (240-335) (STJ22926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain Tissue, 3) Human Brain Tissue, with CCKBR Rabbit pAb diluted at 1:2,000.

Anti-CCKBR antibody (306-356 aa) (STJ197409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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STJ70710 (1µg/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CCKBR antibody (Internal) (STJ70710)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Cow

€390.63

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL1 antibody (20-100 aa) (STJ191421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from RPMI 8226 cells, using CCL1 Rabbit polyclonal antibody (STJ11103818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CCL1 antibody (24-96) (STJ11103818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunofluorescence analysis of A549 cells using CCL11 antibody (STJ29706). Blue: DAPI for nuclear staining.

Anti-CCL11 antibody (1-97) (STJ29706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of HepG2, mouse thymus, mouse skeletal muscle cells using Eotaxin Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CCL11 antibody (41-90 aa) (STJ96809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-CCL11/Eotaxin antibody (26-97 aa) [SRM5287] (STJ11107189)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€161.88 - €718.13

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Anti-CCL11/Eotaxin antibody [CAT-212] (STJA0019033)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/RIA/WB

Reactivity :

Human/Mouse

€473.13 - €2,040.63

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL13 antibody (20-100 aa) (STJ191422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL16 antibody (10-90 aa) (STJ191423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CCL17 antibody (21-70 aa) (STJ194761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CCL17 using CCL17 Rabbit mAb (STJ11107126) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 20s.

Anti-CCL17 antibody (24-94 aa) [SRM5239] (STJ11107126)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of recombinant Human CCL17/TARC Protein, using CCL17 Rabbit polyclonal antibody (STJ22928) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CCL17 antibody (24-94) (STJ22928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of HeLa, using AAK1 Rabbit polyclonal antibody (STJ11104710) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CCL17 antibody (450-550) (STJ11104710)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunofluorescence analysis of paraffin-embedded mouse spleen using CCL19 Rabbit polyclonal antibody (STJ119251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CCL19 antibody (22-95) (STJ119251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of recombinant Human CCL2/MCP-1 Protein, using CCL2/MCP-1 Rabbit polyclonal antibody (STJ11104637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CCL2 antibody (22-99) (STJ11104637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of lysates from A-549 cells, using CCL2/MCP-1 Rabbit monoclonal antibody (STJ11104938) at 1:8000 dilution. A-549 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CCL2 antibody (22-99) [S4938RM] (STJ11104938)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of A-549, using CCL2/MCP-1 antibody (STJ29416) at 1:2000 dilution. A-549 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CCL2 antibody (24-99) (STJ29416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL2 antibody (STJ194973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis using MCP-1 monoclonal antibody against truncated MCP-1 recombinant protein.

Anti-CCL2 antibody [1A7B8] (STJ98233)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€180.00 - €435.00

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Anti-CCL2/MCP-1 antibody [SRM14620] (STJ11109350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using CCL20 antibody (STJ117785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-CCL20 antibody (1-95) (STJ117785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunohistochemistry analysis of CCL21 in paraffin-embedded human stomach using CCL21 Rabbit polyclonal antibody (STJ22930) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CCL21 antibody (24-134) (STJ22930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€140.63 - €655.63

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STJ72226 (0. 1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CCL21 antibody (Internal) (STJ72226)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Anti-CCL22 antibody (1-50) (STJA0010198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CCL22 antibody (31-80) (STJA0010152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CCL23 antibody (22-120 aa) (STJ11105997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL23 antibody (40-120 aa) (STJ191424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CCL25, using CCL25 rabbit polyclonal antibody (STJ116184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-CCL25 antibody (24-150) (STJ116184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL25 antibody (30-110 aa) (STJ191425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of CCL25 in paraffin-embedded Rat large intestine tissue using CCL25 Rabbit polyclonal antibody (STJ11105643) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CCL25 antibody (50-150) (STJ11105643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CCL25 antibody [SRM14927] (STJ11109657)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of PC-3, A549, OVCAR3, mouse colon cells using Eotaxin-3 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CCL26 antibody (31-80 aa) (STJ96515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates using CCL27 Rabbit pAb (STJ111906) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CCL27 antibody (25-112) (STJ111906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of mouse-kidney lysis using CCL27 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-CCL27 antibody (51-100 aa) (STJ97677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CCL28 antibody (20-127 aa) (STJ11107127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€204.38 - €929.38

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Western blot analysis of various lysates, using CCL28 Rabbit polyclonal antibody (STJ22934) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CCL28 antibody (20-127) (STJ22934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL28 antibody (50-130 aa) (STJ191426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of recombinant Human CCL3/MIP-1 alpha Protein using CCL3 Rabbit polyclonal antibody (STJ11105636) at 1:1000 dilution. NA NA Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. NA NA Lysates/proteins: 5 ng/2ng/1ng per lane. NA NA Blocking buffer: 3% nonfat dry milk in TBST. NA NA Detection: ECL Basic Kit. NA NA Exposure time: 10s.

Anti-CCL3 antibody (23-92) (STJ11105636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of lysates from THP-1 cells, using CCL3 Rabbit polyclonal antibody (STJ29705) at 1:1000 dilution. THP-1 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CCL3 antibody (24-92) (STJ29705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CCL3 antibody (C-Term) (STJA0010310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-CCL3/MIP-alpha antibody [SRM13523] (STJ11108253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-CCL3/MIP-alpha antibody [SRM13524] (STJ11108254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of extracts of B-cell cells, using CCL3L1 antibody (STJ22935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CCL3L1 antibody (24-93) (STJ22935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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