• Immunofluorescence analysis of U-2 OS cells using CAMLG Rabbit polyclonal antibody (STJ115674) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using CAMLG Rabbit polyclonal antibody (STJ115674) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CAMLG in paraffin-embedded mouse lung using CAMLG Rabbit polyclonal antibody (STJ115674) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CAMLG in paraffin-embedded human stomach using CAMLG Rabbit polyclonal antibody (STJ115674) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CAMLG Rabbit polyclonal antibody (STJ115674) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CAMLG antibody (1-190) (STJ115674)

SKU:
STJ115674

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAMLG (1-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAMLG
Gene ID: 819
Uniprot ID: CAMLG_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CAMLG (NP_001736.1).
Immunogen Sequence: MESMAVATDGGERPGVPAGS GLSASQRRAELRRRKLLMNS EQRINRIMGFHRPGSGAEEE SQTKSKQQDSDKLNSLSVPS VSKRVVLGDSVSTGTTDQQG GVAEVKGTQLGDKLDSFIKP PECSSDVNLELRQRNRGDLT ADSVQRGSRHGLEQYLSRFE EAMKLRKQLISEKPSQEDGN TTEEFDSFRI
Tissue Specificity Ubiquitous. Highest levels in brain, testis and ovary.
Function Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1/WRB, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. Required for the stability of GET1. Stimulates calcium signaling in T cells through its involvement in elevation of intracellular calcium. Essential for the survival of peripheral follicular B cells.
Protein Name Guided Entry Of Tail-Anchored Proteins Factor Camlg
Calcium Signal-Modulating Cyclophilin Ligand
Database Links Reactome: R-HSA-9609523
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Guided Entry Of Tail-Anchored Proteins Factor Camlg antibody
Anti-Calcium Signal-Modulating Cyclophilin Ligand antibody
Anti-CAMLG antibody
Anti-CAML antibody
Anti-GET2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance