• Western blot analysis of extracts of various cell lines, using CAMK4 antibody (STJ27257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using CAMK4 antibody (STJ27257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human tonsil using CAMK4 rabbit polyclonal antibody (STJ27257) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using CAMK4 rabbit polyclonal antibody (STJ27257) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using CAMK4 rabbit polyclonal antibody (STJ27257) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Neuro-2a cells using CAMK4 rabbit polyclonal antibody (STJ27257) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using CAMK4 rabbit polyclonal antibody (STJ27257) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CAMK4 antibody (304-473) (STJ27257)

SKU:
STJ27257

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-CAMK4 (304-473) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: CAMK4
Gene ID: 814
Uniprot ID: KCC4_HUMAN
Immunogen Region: 304-473
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 304-473 of human CAMK4 (NP_001735.1).
Tissue Specificity Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
Post Translational Modifications Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13. Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation.
Function Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
Protein Name Calcium/Calmodulin-Dependent Protein Kinase Type Iv
Camk Iv
Cam Kinase-Gr
Database Links Reactome: R-HSA-111932
Reactome: R-HSA-2151201
Reactome: R-HSA-442729
Reactome: R-HSA-9022535
Reactome: R-HSA-9022692
Reactome: R-HSA-9617324
Cellular Localisation Cytoplasm
Nucleus
Localized In Hippocampal Neuron Nuclei
In Spermatids
Associated With Chromatin And Nuclear Matrix
Alternative Antibody Names Anti-Calcium/Calmodulin-Dependent Protein Kinase Type Iv antibody
Anti-Camk Iv antibody
Anti-Cam Kinase-Gr antibody
Anti-CAMK4 antibody
Anti-CAMK antibody
Anti-CAMK-GR antibody
Anti-CAMKIV antibody

Information sourced from Uniprot.org

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