Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates, using CD200R1 Rabbit polyclonal antibody (STJ11105432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-CD200R1 antibody (29-240) (STJ11105432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of Mouse spleen, using CD201 antibody (STJ11104990) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD201 antibody (18-210 aa) (STJ11104990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Western blot analysis of K562 cells using CD207 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD207 antibody (61-110 aa) (STJ96640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using Langerin/CD207 antibody (STJ28636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD207 antibody (70-327) (STJ28636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using CD209 Rabbit mAb (STJ11102515) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CD209 antibody (1-100) [S5MR] (STJ11102515)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of mouse brain cells using CD209 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-CD209 antibody (261-310 aa) (STJ97328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of U-937 cells using CD209 Rabbit polyclonal antibody (STJ111093) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD209 antibody (265-404) (STJ111093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-Cd209b antibody [22D1] (STJA0001330)

Clonality :

Monoclonal

Host :

Armenian Hamster

Applications :

FC/IHC-F/WB

Reactivity :

Mouse

€116.88 - €235.63

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Anti-CD210/Il10ra antibody [1B1.3A] (STJA0001418)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/WB/Block

Reactivity :

Mouse

€116.88 - €235.63

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Western blot analysis of various lysates using CD22 Rabbit polyclonal antibody (STJ11105561) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : Jurkat. Exposuretime: 60s.

Anti-CD22 antibody (1-100) (STJ11105561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various lysates using CD22 Rabbit mAb (STJ11107296) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Negative control (NC) : Jurkat<br>Exposure time: 60s.

Anti-CD22 antibody (20-687 aa) [SRM] (STJ11107296)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Immunofluorescence analysis of Raji cells using CD22 Rabbit polyclonal antibody (STJ28354) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD22 antibody (708-847) (STJ28354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of 293 cells using CD22 Polyclonal Antibody

Anti-CD22 antibody (776-825 aa) (STJ92105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CD22 antibody [MEM-01] (STJ16100034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Human/Non-Human Primates

€506.88

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Anti-CD22 antibody [SRM] (STJ11107213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Anti-CD221 IGF Receptor antibody [1H7] (STJ16101843)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/FC/WB

Reactivity :

Human

€2,111.88

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Anti-CD222 antibody [MEM-238] (STJ16100179)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Non-Human Primates/Human

€571.88

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Western blot analysis of various lysates using CD226 Rabbit pAb (STJ115360) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CD226 antibody (19-336) (STJ115360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of COS7 cells using DNAM-1 Polyclonal Antibody diluted at 1：500

Anti-CD226 antibody (287-336 aa) (STJ92746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€60.00 - €315.00

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Western blot analysis of HeLa cells using DNAM-1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD226 antibody (71-120 aa) (STJ96767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates, using CD226/DNAM-1 Rabbit monoclonal antibody (STJ11105046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD226 antibody [S5046RM] (STJ11105046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Anti-CD227 antibody [C595] (STJ16100530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC/IHC-F/ELISA/IF

Reactivity :

Human

€1,173.13

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Anti-CD239 antibody [BRIC221] (STJ16100542)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA/IF/FC/IHC-F

Reactivity :

Human/Pig

€926.88

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Immunohistochemistry analysis of paraffin-embedded human oophoroma using CD239/BCAM Rabbit monoclonal antibody (STJ11103094) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD239/BCAM antibody (529-628) [S4MR] (STJ11103094)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Anti-CD24 antibody (1-80 aa) [SRM] (STJ11107235)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD24 Rabbit polyclonal antibody (STJ22979) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD24 antibody (1-80) (STJ22979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysate from NIH/3T3 cells, using CD24 antibody.

Anti-CD24 antibody (14-63 aa) (STJ92106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from Mouse liver using mouse CD24 Rabbit polyclonal antibody (STJ11105518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD24 antibody (27-53) (STJ11105518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Anti-CD24 antibody (30-70 aa) (STJ13100877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

€414.38

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Western blot analysis of KB cells using CD244 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD244 antibody (101-150 aa) (STJ97310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from U-937 cells using CD244 Rabbit mAb (STJ11107322) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 180s.

Anti-CD244 antibody (22-221 aa) [SRM] (STJ11107322)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of various lysates, using CD244 antibody (STJ22980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD244 antibody (22-224) (STJ22980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-CD244 antibody (Extracellular region) [M053] (STJA0005334)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human

€488.13

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Western blot analysis of lysates from Jurkat cells, treated with UV 15', using CD3 zeta Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CD247 antibody (111-160 aa) (STJ92112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Immunofluorescence analysis of A549 cells using CD3H antibody (STJ22982). Blue: DAPI for nuclear staining.

Anti-CD247 antibody (50-164) (STJ22982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Flow cytometry:1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3H Rabbit monoclonal antibody (STJ11101284, 10 Mu g/mL, orange line) or Rabbit IgG isotype control ( 10 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-CD247 antibody (65-164) [S4MR] (STJ11101284)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis using CD247 monoclonal antibody against CD247-hIgGFc transfected HEK293 cell lysate.

Anti-CD247 antibody [4B10] (STJ97916)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis of extracts of various cell lines, using CD248 antibody (STJ114576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from HeLa cells, using CD248 Rabbit polyclonal antibody (STJ114579) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of HepG2 cells using CD248 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD248 antibody (341-390 aa) (STJ96632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CD25/IL2RA antibody [143-13] (STJ16102559)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FUNC/WB/IF/IHC

Reactivity :

Human

€359.38 - €1,655.63

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Anti-CD26 antibody [202-36] (STJ16102562)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human/Rat

€359.38 - €1,655.63

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Anti-CD26 antibody [202.36] (STJ16100026)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IF/WB

Reactivity :

Human/Rat

€471.88

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Anti-CD26 antibody [5+00E+08] (STJ16100784)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/IP/WB

Reactivity :

Rat

€828.13

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Confocal imaging of human tonsil using CD27 Rabbit monoclonal antibody (STJ11101350, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-CD27 antibody (1-100) [S0MR] (STJ11101350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

€161.88 - €718.13

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD27 antibody (1-80 aa) (STJ191672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-CD27 (PT1818) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: K562Predicted band size: 29kDaObserved band size: 40kDa

Anti-CD27 antibody (160-260) [PT1818] (STJA0006232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€60.00 - €315.00

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STJ73070 (0. 3µg/ml) staining of Human Spleen (A) , Tonsil (B) and Thymus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD27 antibody (161-175) (STJ73070)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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