• Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CACYBP antibody (1-228) (STJ11100880)

SKU:
STJ11100880

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CACYBP (1-228) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CACYBP
Gene ID: 27101
Uniprot ID: CYBP_HUMAN
Immunogen Region: 1-228
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CACYBP (NP_055227.1).
Immunogen Sequence: MASEELQKDLEEVKVLLEKA TRKRVRDALTAEKSKIETEI KNKMQQKSQKKAELLDNEKP AAVVAPITTGYTVKISNYGW DQSDKFVKIYITLTGVHQVP TENVQVHFTERSFDLLVKNL NGKSYSMIVNNLLKPISVEG SSKKVKTDTVLILCRKKVEN TRWDYLTQVEKECKEKEKPS YDTETDPSEGLMNVLKKIYE DGDDDMKRTINKAWVESREK QAKGDTEF
Post Translational Modifications Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
Function May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
Protein Name Calcyclin-Binding Protein
Cacybp
Hcacybp
S100a6-Binding Protein
Siah-Interacting Protein
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic At Low Calcium Concentrations
In Neuroblastoma Cells
After A Retinoic Acid (Ra) Induction And Calcium Increase
It Localizes In Both The Nucleus And Cytoplasm
The Nuclear Fraction May Be Phosphorylated
Alternative Antibody Names Anti-Calcyclin-Binding Protein antibody
Anti-Cacybp antibody
Anti-Hcacybp antibody
Anti-S100a6-Binding Protein antibody
Anti-Siah-Interacting Protein antibody
Anti-CACYBP antibody
Anti-S100A6BP antibody
Anti-SIP antibody
Anti-PNAS-107 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance