Immunofluorescence Antibodies | St John's Laboratory

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3-K27 antibody (STJ23988) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Di-Methyl-Histone H3-K27 antibody (16-37) (STJ23988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of HeLa, using DiMethyl-Histone H3-K36 antibody (STJ23991) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Di-Methyl-Histone H3-K36 antibody (Around K36) (STJ23991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody (STJ23982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Di-Methyl-Histone H3-K4 antibody (2-19) (STJ23982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

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Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and DiMethyl-Histone H3-K79 rabbit polyclonal antibody (STJ23994). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Di-Methyl-Histone H3-K79 antibody (STJ23994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of HeLa, using DiMethyl-Histone H3-K9 antibody (STJ23985) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Di-Methyl-Histone H3-K9 antibody (2-19) (STJ23985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of NIH-3T3 cells using Di-Methyl-Histone H3 (K10) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Di-Methyl-Histone H3-Lys10 antibody (STJ97223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of MCF-7 cells using Di-Methyl-Histone H3 (K5) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Di-Methyl-Histone H3-Lys5 antibody (STJ90112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23965) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R17 antibody (2-22) (STJ23965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23967). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R17 antibody (Around Arg17) (STJ23967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Immunofluorescence analysis of U2OS cells using Symmetric DiMethyl-Histone H3-R2 Rabbit polyclonal antibody (STJ23996) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R2 antibody (2-19) (STJ23996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody (STJ24011) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Di-Methyl-Histone H3-R2 antibody (Around R2) (STJ24011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R26 Rabbit polyclonal antibody (STJ23963). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R26 antibody (Around Arg26) (STJ23963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of U-2 OS cells using Symmetric DiMethyl-Histone H3-R26 Rabbit polyclonal antibody (STJ23968) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R26 antibody (Around Arg26) (STJ23968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R8 Rabbit polyclonal antibody (STJ23997). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R8 antibody (Around R8) (STJ23997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R8 antibody (STJ24013) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Di-Methyl-Histone H3-R8 antibody (Around R8) (STJ24013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of 293T cells using DiMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23971). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H4-K20 antibody (Around K20) (STJ23971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H4-R3 antibody (2-19) (STJ23979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23973). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H4-R3 antibody (Around Arg3) (STJ23973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Immunofluorescence analysis of human-breast-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Di-Methyl-TP53-Lys370 antibody (STJ90115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of MCF-7 cells using Smac/Diablo Rabbit pAb (STJ115393). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DIABLO antibody (1-232) (STJ115393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various lysates using Smac/Diablo Rabbit mAb (STJ11102390) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-DIABLO antibody (56-239) [S0MR] (STJ11102390)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using DIAPH1 Rabbit pAb (STJ28339) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-DIAPH1 antibody (1149-1263) (STJ28339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of 293F, using DICER1 antibody (STJ118244) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-DICER1 antibody (1100-1200) (STJ118244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using DICER1 Rabbit pAb (STJ27817) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-DICER1 antibody (1200-1460) (STJ27817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using Dio-1 Polyclonal Antibody diluted at 1：2000

Anti-DIDO1 antibody (161-210 aa) (STJ92716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-DiMethyl-Histone H3-Lys79 antibody [PT0928R] (STJA0039842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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STJ71102 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-DIO2 antibody (Internal) (STJ71102)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse/Rat/Pig

$421.88

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Immunofluorescence analysis of L929 cells using DIP2C Rabbit polyclonal antibody (STJ11100306) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DIP2C antibody (100-260) (STJ11100306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various cells using Di-Ras1 Polyclonal Antibody

Anti-DIRAS1 antibody (149-198 aa) (STJ92718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunocytochemistry analysis of DISC1 (green) in Hela using DISC1 antibody, and DAPI (blue)

Anti-DISC1 antibody (1-100 aa) [R03-7F5] (STJA0011237)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$332.78

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Immunofluorescence analysis of U-2 OS cells using DISC1 Rabbit monoclonal antibody (STJ11102128) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DISC1 antibody (1-100) [S8MR] (STJ11102128)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Anti-Disemboided antibody (STJ500762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

$629.78

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Anti-Disemboided antibody (STJ500763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

$629.78

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Anti-Disemboided antibody {Biotin} (STJ500764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

$713.48

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Anti-Disemboided antibody {Biotin} (STJ500766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

$713.48

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Anti-Disemboided antibody {FITC} (STJ500765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

$713.48

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Anti-Disemboided antibody {FITC} (STJ500767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

$713.48

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STJ70518 (2. 5ug/ml) staining of U87. Detected by immunofluorescence.

Anti-Dispatched homolog 1 antibody (C-Term) (STJ70518)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Pig

$421.88

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Immunofluorescence analysis of U-2 OS cells using DKC1 Rabbit polyclonal antibody (STJ114780) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DKC1 antibody (1-180) (STJ114780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of HeLa cells using Dyskerin Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DKC1 antibody (171-220 aa) (STJ92804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of U2OS cells using DKC1 antibody (STJ23384). Blue: DAPI for nuclear staining.

Anti-DKC1 antibody (245-514) (STJ23384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunocytochemistry analysis of DKC1 (green) in K562 using DKC1 antibody, and DAPI (blue).

Anti-DKC1 antibody (371-384 aa) [R02-2D8] (STJA0011240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$357.08

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Anti-Dkk-1 antibody [PT1237R] (STJA0040152)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot analysis of Hela cells using Dkk-1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-DKK1 antibody (181-230 aa) (STJ97257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ70585 (1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-DKK1 antibody (C-Term) (STJ70585)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC/FC

Reactivity :

Human/Mouse/Cow

$421.88

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Immunofluorescence analysis of SH-SY5Y cells using DKK3 Rabbit monoclonal antibody (STJ11101965) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DKK3 antibody (200-350) [S5MR] (STJ11101965)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of extracts of various cell lines, using DKK3 antibody (STJ11102858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DKK3 antibody (22-200) (STJ11102858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of MCF7 cells using DKK3 Rabbit polyclonal antibody (STJ23387) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DKK3 antibody (22-200) (STJ23387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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