• Immunofluorescence analysis of C6 cells using COMMD5 antibody (STJ118945) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using COMMD5 Rabbit polyclonal antibody (STJ118945) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COMMD5 antibody (1-224) (STJ118945)

SKU:
STJ118945

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COMMD5 (1-224) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COMMD5
Gene ID: 28991
Uniprot ID: COMD5_HUMAN
Immunogen Region: 1-224
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human COMMD5 (NP_054785.2).
Immunogen Sequence: MSAVGAATPYLHHPGDSHSG RVSFLGAQLPPEVAAMARLL GDLDRSTFRKLLKFVVSSLQ GEDCREAVQRLGVSANLPEE QLGALLAGMHTLLQQALRLP PTSLKPDTFRDQLQELCIPQ DLVGDLASVVFGSQRPLLDS VAQQQGAWLPHVADFRWRVD VAISTSALARSLQPSVLMQL KLSDGSAYRFEVPTAKFQEL RYSVALVLKEMADLEKRCER RLQD
Tissue Specificity Highly expressed in heart, stomach, jejunum, kidney, liver, and adrenal gland. Expression was generally higher in adult organs than in fetal tissues, particularly in heart, kidney, and liver.
Function May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. Negatively regulates cell proliferation. Negatively regulates cell cycle G2/M phase transition probably by transactivating p21/CDKN1A through the p53/TP53-independent signaling pathway. Involved in kidney proximal tubule morphogenesis. Down-regulates activation of NF-kappa-B.
Protein Name Comm Domain-Containing Protein 5
Hypertension-Related Calcium-Regulated Gene Protein
Hcarg
Database Links Reactome: R-HSA-8951664
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Comm Domain-Containing Protein 5 antibody
Anti-Hypertension-Related Calcium-Regulated Gene Protein antibody
Anti-Hcarg antibody
Anti-COMMD5 antibody
Anti-HT002 antibody

Information sourced from Uniprot.org

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