Anti-Collagen IV antibody [SRM14864] (STJ11109594)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Collagen IV antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14864 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:3000IF/ICC, 1:50-1:200ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COL4A1COL4A3128212851284CO4A1_HUMANCO4A3_HUMANCO4A2_HUMAN |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | P02462 - Highly expressed in placenta.Q01955 - Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.P08572 - NAP29400 - Isoform 2 is found in kidney.P02462 - Highly expressed in placenta.Q01955 - Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.P08572 - NAP29400 - Isoform 2 is found in kidney.P02462 - Highly expressed in placenta.Q01955 - Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.P08572 - NAP29400 - Isoform 2 is found in kidney. |
| Alternative Antibody Names | Anti-Collagen Alpha-1(Iv Chain Cleaved Into - Arresten antibodyAnti-COL4A1 antibodyAnti-Collagen Alpha-3(Iv Chain antibodyAnti-Goodpasture Antigen Cleaved Into - Tumstatin antibodyAnti-COL4A3 antibodyAnti-Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin antibodyAnti-COL4A2 antibodyAnti-Collagen Alpha-5(Iv Chain antibodyAnti-COL4A5 antibodyAnti-Collagen Alpha-1(Iv Chain Cleaved Into - Arresten antibodyAnti-COL4A1 antibodyAnti-Collagen Alpha-3(Iv Chain antibodyAnti-Goodpasture Antigen Cleaved Into - Tumstatin antibodyAnti-COL4A3 antibodyAnti-Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin antibodyAnti-COL4A2 antibodyAnti-Collagen Alpha-5(Iv Chain antibodyAnti-COL4A5 antibodyAnti-Collagen Alpha-1(Iv Chain Cleaved Into - Arresten antibodyAnti-COL4A1 antibodyAnti-Collagen Alpha-3(Iv Chain antibodyAnti-Goodpasture Antigen Cleaved Into - Tumstatin antibodyAnti-COL4A3 antibodyAnti-Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin antibodyAnti-COL4A2 antibodyAnti-Collagen Alpha-5(Iv Chain antibodyAnti-COL4A5 antibody |
Information sourced from Uniprot.org