Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-LRRK2 antibody (STJ191973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-LRRK2 antibody [PT0940R] (STJA0040231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-LRRK2 antibody [R05-6E9] (STJA0035794)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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STJ70619 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue).

Anti-LRRK2/PARK8 antibody (Internal) (STJ70619)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human

£312.50

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STJ73586 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-LYP/PTPN22 antibody (Internal) (STJ73586)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Chimpanzee

£312.50

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Anti-Lysophospholipase 1 antibody [R03-3R1] (STJA0037056)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-MonoMethyl-Histone H2B antibody [R96-4G-1] (STJA0035527)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of PC-12 cells using MTAP Rabbit polyclonal antibody (STJ11104375) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-MTAP antibody (1-283) (STJ11104375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-MTAP antibody [SRM13106] (STJ11107836)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug mTOR Rabbit polyclonal antibody (STJ29884 1:70). Western blot was performed from the immunoprecipitate using mTOR Rabbit polyclonal antibody (STJ29884) at a dilition of 1:500.

Anti-mTOR antibody (1-300) (STJ29884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using mTOR antibody (STJ113771) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-mTOR antibody (2400-2500) (STJ113771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various cells using mTOR Polyclonal Antibody

Anti-MTOR antibody (2447-2496) (STJ94278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig

£49.00 - £261.00

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Anti-mTOR antibody [PT0351R] (STJA0039470)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-mTOR antibody [R07-8H4] (STJA0035711)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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STJ73757 Immunofluorescence analysis of paraformaldehyde fixe HEK293 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-MTOR/FRAP2 antibody (1137-1150) (STJ73757)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ73725 Immunofluorescence analysis of paraformaldehyde fixe HEK293 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-MTOR/FRAP2 antibody (C-Term) (STJ73725)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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Anti-N-Acetyl-D-Glucosamine Kinase/NAGK antibody (1-344aa) [AT4D12] (STJA0041632)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated antibody (STJA0040898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/WB

Reactivity :

Human/Other Mammals/Rat

£528.50

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Anti-Neurofilament heavy polypeptide, phosphorylated antibody (STJA0040651)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IF/ICC/IHC/WB

Reactivity :

Bovine/Dog/Horse/Human/Mouse/Other Mammals/Pig/Rat

£528.50

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Anti-Neurofilament heavy polypeptide, phosphorylated antibody (STJA0040897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/IHC/WB

Reactivity :

Bovine/Horse/Human/Mouse/Other Mammals/Pig/Rat

£528.50

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Anti-Neurofilament heavy polypeptide, phosphorylated antibody [NAP4] (STJA0040751)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/ICC/IHC/WB

Reactivity :

Bovine/Chicken/Horse/Human/Mouse/Other Mammals/Pig/Rat

£528.50

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Immunofluorescence analysis of HUVEC cells, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NIFK antibody (200-249) (STJ94492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using NOD2 Rabbit polyclonal antibody (STJ11105602) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOD2 antibody (28-270) (STJ11105602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of PC-12 cells using NOD2 Rabbit polyclonal antibody (STJ118437) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOD2 antibody (30-1040) (STJ118437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-NOLC1 antibody [SRM13156] (STJ11107886)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ24784) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1000-1203) (STJ24784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, NOS3 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-NOS3 antibody (1145-1194) (STJ94539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues with DAB staining using NOS3 monoclonal antibody.

Anti-NOS3 antibody [6H2] (STJ98282)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Confocal imaging of paraffin-embedded mouse heart using NPPA Rabbit monoclonal antibody (STJ11104694, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-NPPA antibody (1-100) [S4694RM] (STJ11104694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Western blot analysis of various lysates using NPPA Rabbit polyclonal antibody (STJ24798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NPPA antibody (26-151) (STJ24798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of PC-12 cells using Rev-Erb Alpha/NR1D1 Rabbit polyclonal antibody (STJ11100546) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NR1D1 antibody (280-360) (STJ11100546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunocytochemistry analysis of NR1D1 (green) in Hela using NR1D1 antibody, and DAPI (blue)

Anti-NR1D1 antibody (591-605 aa) [R05-8B1] (STJA0011699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-NR1D1 antibody [PT1219R] (STJA0040117)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-NR1D1 antibody [R02-1H2] (STJA0036803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of HeLa cells using NUR77 Rabbit polyclonal antibody (STJ11103838) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NR4A1 antibody (200-300) (STJ11103838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using Nur77 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NR4A1 antibody (317-366) (STJ94578)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-NSE antibody [4C7-4F10-10F9] (STJA0032779)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-NSE antibody [SM5003] (STJA0005003)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Western blot analysis of NSE at dilution of 1:1000

Anti-NSE antibody [SM5098] (STJA0005098)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunofluorescence analysis of PC-12 cells using OGT Rabbit polyclonal antibody (STJ24862) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-OGT antibody (510-699) (STJ24862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ71101 (0. 05µg/ml) staining of Rat Pancreas lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-OGT antibody (Internal) (STJ71101)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-OGT antibody [PT1337R] (STJA0040061)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-OGT antibody [R05-5C2] (STJA0034663)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-OGT antibody [R08-6W9] (STJA0036699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Oxidized Phospholipid antibody [E06] (STJA0032097)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FCM/FuncS/IF/IHC/RIA/WB

Reactivity :

Human/Mouse/Rabbit

£367.50

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine pAb (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody (STJ11101040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Pan-Phospho-Tyrosine antibody [PY20] (STJA0019177)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Broad-Spectrum

£209.50 - £1,333.50

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine Mouse mAb (STJ11101091) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody [S1MM] (STJ11101091)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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