Anti-LYP/PTPN22 antibody (Internal) (STJ73586)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-LYP/PTPN22 (Internal) is suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/FC/IF/IHC |
| Host | Goat |
| Reactivity | Human/Chimpanzee |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments showed a 100kDa band in some Daudi cell lysates at 0.5-1ug/ml. This molecular weight is routinely observed by other sources. An additional band at 75kDa was als |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PTPN22 |
| Gene ID | 26191 |
| Uniprot ID | PTN22_HUMAN |
| Accession Number | NP_057051; NP_036543.4; NP_001180360.1; NP_001295226.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | CPPNKPAESVQSNNS |
| Specificity | This antibody is specific for human LYP1 and will not cross-react with LYP2. |
Additional Info
| Post Translational Modifications | Phosphorylation on Ser-35 by PKC/PRKCD abrogates its ability to dephosphorylate and inactivate the SRC family kinases. |
| Function | Acts as a negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR)-induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon. Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy. Acts as an activator of NLRP3 inflammasome assembly by mediating dephosphorylation of 'Tyr-861' of NLRP3. Dephosphorylates phospho-anandamide (p-AEA), an endocannabinoid to anandamide (also called N-arachidonoylethanolamide). |
| Protein Name | Tyrosine-Protein Phosphatase Non-Receptor Type 22Hematopoietic Cell Protein-Tyrosine Phosphatase 70z-PepLymphoid PhosphataseLypPest-Domain PhosphatasePep |
| Database Links | Reactome: R-HSA-202427Reactome: R-HSA-202430 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 22 antibodyAnti-Hematopoietic Cell Protein-Tyrosine Phosphatase 70z-Pep antibodyAnti-Lymphoid Phosphatase antibodyAnti-Lyp antibodyAnti-Pest-Domain Phosphatase antibodyAnti-Pep antibodyAnti-PTPN22 antibodyAnti-PTPN8 antibody |
Information sourced from Uniprot.org