• STJ73586 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73586 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-LYP/PTPN22 antibody (Internal) (STJ73586)

SKU:
STJ73586-100

€379.38
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Host: Goat
Applications: Pep-ELISA/FC/IF/IHC
Reactivity: Human/Chimpanzee
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-LYP/PTPN22 (Internal) is suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the nuclei of U2OS cells. 10µg/ml
FC-Flow cytometric analysis of Jurkat cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PTPN22
Gene ID: 26191
Uniprot ID: PTN22_HUMAN
Immunogen Region: Internal
Accession Number: NP_057051; NP_036543.4; NP_001180360.1; NP_001295226.1
Specificity: This antibody is specific for human LYP1 and will not cross-react with LYP2.
Immunogen Sequence: CPPNKPAESVQSNNS
Post Translational Modifications Phosphorylation on Ser-35 by PKC/PRKCD abrogates its ability to dephosphorylate and inactivate the SRC family kinases.
Function Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR)-induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon. Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy. Acts as an activator of NLRP3 inflammasome assembly by mediating dephosphorylation of 'Tyr-861' of NLRP3. Dephosphorylates phospho-anandamide (p-AEA), an endocannabinoid to anandamide (also called N-arachidonoylethanolamide).
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 22
Hematopoietic Cell Protein-Tyrosine Phosphatase 70z-Pep
Lymphoid Phosphatase
Lyp
Pest-Domain Phosphatase
Pep
Database Links Reactome: R-HSA-202427
Reactome: R-HSA-202430
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 22 antibody
Anti-Hematopoietic Cell Protein-Tyrosine Phosphatase 70z-Pep antibody
Anti-Lymphoid Phosphatase antibody
Anti-Lyp antibody
Anti-Pest-Domain Phosphatase antibody
Anti-Pep antibody
Anti-PTPN22 antibody
Anti-PTPN8 antibody

Information sourced from Uniprot.org

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