Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of NIH-3T3 cells using Nesprin 1 Rabbit monoclonal antibody (STJ11103130) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Nesprin 1 antibody (8200-8400) [S0MR] (STJ11103130)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of NIH/3T3 cells using Nesprin 1 Rabbit polyclonal antibody (STJ11103244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Nesprin 1 antibody (8220-8410) (STJ11103244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Nestin antibody [SM0437] (STJA0010437)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Immunohistochemistry analysis of Nestin. Sample: Human kidney, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-Nestin antibody [SM4923] (STJA0004923)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunohistochemistry analysis of nestin. Sample: Human glioblastoma multiforme, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-Nestin antibody [SM4950] (STJA0004950)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Immunofluorescence analysis of HeLa cells using NET1 antibody (STJ24743). Blue: DAPI for nuclear staining.

Anti-NET1 antibody (1-300) (STJ24743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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STJ70811 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and secreted staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Netrin 1 antibody (C-Term) (STJ70811)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Immunofluorescence analysis of NIH/3T3 cells using NEU3 Rabbit polyclonal antibody (STJ115782) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NEU3 antibody (222-461) (STJ115782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using NeuN Mouse mAb (STJ11107255) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-NeuN antibody [SMM] (STJ11107255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-NEURL1 antibody (219-268) (STJ94417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Neuralized-2 Polyclonal Antibody

Anti-NEURL2 antibody (99-148) (STJ94418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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STJ70314 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Neuro-d4/DPF1 antibody (C-Term) (STJ70314)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Anti-Neurochondrin antibody (STJ501948)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£466.50

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Anti-Neurochondrin antibody {Biotin} (STJ501949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemistry of mouse tissue with Anti-Neurochondrin primary antibody (FITC) (STJ501950) at 1:50 dilution in buffer.

Anti-Neurochondrin antibody {FITC} (STJ501950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of paraffin-embedded mouse brain using NEUROD1 Rabbit pAb (STJ11107005) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-NEUROD1 antibody (1-136 aa) (STJ11107005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Neuro D2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NEUROD2 antibody (61-110) (STJ94427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Neurofilament antibody [402] (STJ16100254)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/IF/WB

Reactivity :

Bovine/Guinea Pig/Human/Rat

£495.50

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Anti-Neurofilament antibody [403] (STJ16100255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/IF/WB

Reactivity :

Bovine/Guinea Pig/Human/Rat

£521.50

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Anti-Neurofilament antibody [RNF403] (STJ16100223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC-F/IHC-P/WB

Reactivity :

Human/Hamster/Monkey/Rat/Xenopus

£535.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using NF200 at dilution of 1:4000

Anti-Neurofilament heavy polypeptide antibody [SM4956] (STJA0004956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Mouse

£170.50 - £233.50

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Western blot analysis of NF200. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-Neurofilament heavy polypeptide antibody [SM4961] (STJA0004961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Confocal imaging of paraffin-embedded Mouse brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Neurofilament L antibody (60-250) [S0MR] (STJ11103250)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ70748 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Neuroligin 1 antibody (Internal) (STJ70748)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ71114 (2µg/ml) staining of Human Pancrease lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Neuroligin 2 antibody (Internal) (STJ71114)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Anti-Neurturin antibody (STJA0002881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Anti-Neurturin antibody [1B11] (STJA0002884)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Anti-Neurturin antibody [3D12] (STJA0002882)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Anti-Neurturin antibody [4G8] (STJA0002883)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Western blot analysis of various lysates using Neutrophil Elastase (ELANE) Rabbit mAb (STJ11103639) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Neutrophil Elastase antibody (167-267) [S9MR] (STJ11103639)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using NEXN Rabbit polyclonal antibody (STJ115102) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NEXN antibody (516-675) (STJ115102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of SKOV3 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ114897). Western blot was performed from the immunoprecipitate using NF2 antibody (STJ114897) at a dilution of 1:1000.

Anti-NF2 antibody (477-576) (STJ114897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using NF2 Polyclonal Antibody diluted at 1：500

Anti-NF2 antibody (485-534) (STJ94445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 823 293T-UV HELA Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-NFAT5 antibody (212-284 aa) (STJ98961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Jurkat cells using NFAT2 Rabbit polyclonal antibody (STJ24751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NFATC1 antibody (20-300) (STJ24751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HuvEc cells using NFATc1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NFATC1 antibody (881-930 aa) (STJ96746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of rat spleen using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFATC2 antibody (1-300) (STJ24752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-colon, antibody was diluted at 1:100

Anti-NFATC2 antibody (640-700 aa) (STJ98986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NFATC3 antibody (STJ28749) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFATC3 antibody (1-290) (STJ28749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using NFATc3 Polyclonal Antibody

Anti-NFATC3 antibody (131-180) (STJ94448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using NFATc4 Polyclonal Antibody diluted at 1：500

Anti-NFATC4 antibody (642-691) (STJ94450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using NFE2L1 Rabbit polyclonal antibody (STJ116953) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NFE2L1 antibody (515-772) (STJ116953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of NRF2 in paraffin-embedded human placenta using NRF2 Rabbit polyclonal antibody (STJ113550) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NFE2L2 antibody (243-400) (STJ113550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from NIH/3T3 cells using NRF2 Rabbit pAb (STJ113718) at 1:1000 dilution incubated overnight at 4 °C. NIH/3T3 cells were treated by MG132 (10 Mu M) at 37 °C for 90 minutes. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 45s.

Anti-NFE2L2 antibody (366-605) (STJ113718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Nrf2 Polyclonal Antibody diluted at 1：1000

Anti-NFE2L2 antibody (556-605) (STJ94554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NFE4 antibody (STJ193183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from rat brain and HUVEC cells, using Nuclear Factor 1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-NFIA antibody (11-60) (STJ94443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of mouse brain, using NFIA antibody (STJ24756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NFIA antibody (180-330) (STJ24756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options