Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of paraffin-embedded mouse brain using NEUROD1 Rabbit pAb (STJ11107005) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-NEUROD1 antibody (1-136 aa) (STJ11107005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using Neuro D2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NEUROD2 antibody (61-110) (STJ94427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Neurofilament antibody [402] (STJ16100254)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/IF/WB

Reactivity :

Bovine/Guinea Pig/Human/Rat

€619.38

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Anti-Neurofilament antibody [403] (STJ16100255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/IF/WB

Reactivity :

Bovine/Guinea Pig/Human/Rat

€651.88

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Anti-Neurofilament antibody [RNF403] (STJ16100223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC-F/IHC-P/WB

Reactivity :

Human/Hamster/Monkey/Rat/Xenopus

€669.38

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Immunohistochemistry analysis of paraffin-embedded mouse brain using NF200 at dilution of 1:4000

Anti-Neurofilament heavy polypeptide antibody [SM4956] (STJA0004956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Mouse

€213.13 - €291.88

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Western blot analysis of NF200. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-Neurofilament heavy polypeptide antibody [SM4961] (STJA0004961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

€213.13 - €291.88

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Confocal imaging of paraffin-embedded Mouse brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Neurofilament L antibody (60-250) [S0MR] (STJ11103250)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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STJ70748 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Neuroligin 1 antibody (Internal) (STJ70748)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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STJ71114 (2µg/ml) staining of Human Pancrease lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Neuroligin 2 antibody (Internal) (STJ71114)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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Anti-Neurturin antibody (STJA0002881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IF

Reactivity :

Human

€548.13 - €3,128.13

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Anti-Neurturin antibody [1B11] (STJA0002884)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

Human

€548.13 - €3,128.13

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Anti-Neurturin antibody [3D12] (STJA0002882)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

Human

€548.13 - €3,128.13

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Anti-Neurturin antibody [4G8] (STJA0002883)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

Human

€548.13 - €3,128.13

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Western blot analysis of various lysates using Neutrophil Elastase (ELANE) Rabbit mAb (STJ11103639) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Neutrophil Elastase antibody (167-267) [S9MR] (STJ11103639)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Immunofluorescence analysis of U-2 OS cells using NEXN Rabbit polyclonal antibody (STJ115102) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NEXN antibody (516-675) (STJ115102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunoprecipitation analysis of 200 Mu g extracts of SKOV3 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ114897). Western blot was performed from the immunoprecipitate using NF2 antibody (STJ114897) at a dilution of 1:1000.

Anti-NF2 antibody (477-576) (STJ114897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various cells using NF2 Polyclonal Antibody diluted at 1：500

Anti-NF2 antibody (485-534) (STJ94445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 823 293T-UV HELA Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-NFAT5 antibody (212-284 aa) (STJ98961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of Jurkat cells using NFAT2 Rabbit polyclonal antibody (STJ24751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NFATC1 antibody (20-300) (STJ24751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of HuvEc cells using NFATc1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NFATC1 antibody (881-930 aa) (STJ96746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of rat spleen using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFATC2 antibody (1-300) (STJ24752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemical analysis of paraffin-embedded Human-colon, antibody was diluted at 1:100

Anti-NFATC2 antibody (640-700 aa) (STJ98986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using NFATC3 antibody (STJ28749) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFATC3 antibody (1-290) (STJ28749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using NFATc3 Polyclonal Antibody

Anti-NFATC3 antibody (131-180) (STJ94448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using NFATc4 Polyclonal Antibody diluted at 1：500

Anti-NFATC4 antibody (642-691) (STJ94450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells using NFE2L1 Rabbit polyclonal antibody (STJ116953) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NFE2L1 antibody (515-772) (STJ116953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of NRF2 in paraffin-embedded human placenta using NRF2 Rabbit polyclonal antibody (STJ113550) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NFE2L2 antibody (243-400) (STJ113550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from NIH/3T3 cells using NRF2 Rabbit pAb (STJ113718) at 1:1000 dilution incubated overnight at 4 °C. NIH/3T3 cells were treated by MG132 (10 Mu M) at 37 °C for 90 minutes. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 45s.

Anti-NFE2L2 antibody (366-605) (STJ113718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using Nrf2 Polyclonal Antibody diluted at 1：1000

Anti-NFE2L2 antibody (556-605) (STJ94554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NFE4 antibody (STJ193183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from rat brain and HUVEC cells, using Nuclear Factor 1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-NFIA antibody (11-60) (STJ94443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of mouse brain, using NFIA antibody (STJ24756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NFIA antibody (180-330) (STJ24756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody (STJ115964). Blue: DAPI for nuclear staining.

Anti-NFIL3 antibody (133-462) (STJ115964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Western blot analysis of various cells using E4BP4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NFIL3 antibody (61-110 aa) (STJ92812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, NF Kappa B-p105/p50 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-NFKB1 antibody (304-353) (STJ94466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of mouse lung, using NFKB1 antibody (STJ28750) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-NFKB1 antibody (41-365) (STJ28750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of BALB-3T3 cells using NFKB1 Rabbit polyclonal antibody (STJ116954) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFKB1 antibody (41-968) (STJ116954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunoprecipitation analysis of 150 Mu g extracts of MCF-7 cells using 3 Mu g NFKB1 antibody (STJ113719). Western blot was performed from the immunoprecipitate using NFKB1 antibody (STJ113719) at a dilution of 1:500.

Anti-NFKB1 antibody (740-964) (STJ113719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various cells using NF Kappa B-p105 Polyclonal Antibody

Anti-NFKB1 antibody (896-945) (STJ94463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis using NF Kappa B-p105/p50 monoclonal antibody against K562 (1) , Jurkat (2) , A431 (3) , HeLa (4) , THP-1 (5) and MCF-7 (6) cell lysate.

Anti-NFKB1 antibody [5D10] (STJ98275)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

€180.00 - €435.00

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Anti-NFKB1 antibody [5D10D11] (STJ98274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

€180.00 - €435.00

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Immunofluorescence analysis of U2OS cells using NF-Kappa B2 antibody (STJ113721) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NFKB2 antibody (690-899) (STJ113721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunofluorescence analysis of U2OS cells using NF-Kappa B2 antibody (STJ24762) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NFKB2 antibody (690-899) (STJ24762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using NF-Kappa B2 Rabbit polyclonal antibody (STJ27537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFKB2 antibody (730-900) (STJ27537)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of Hela cell. 1, NF Kappa B-p100 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). ATG5 mouse monoclonal antibody (3C7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-NFKB2 antibody (833-882) (STJ94458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using I Kappa B Alpha antibody (STJ112997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.

Anti-NFKBIA antibody (1-317) (STJ112997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IkappaB-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NFKBIA antibody (15-64 aa) (STJ93784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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