• Western blot analysis of extracts of various cell lines, using KLK10 antibody (STJ28481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of MCF7 cells using KLK10 rabbit polyclonal antibody (STJ28481) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using KLK10 rabbit polyclonal antibody (STJ28481) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-3 cells using KLK10 rabbit polyclonal antibody (STJ28481) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KLK10 antibody (43-276) (STJ28481)

SKU:
STJ28481

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLK10 (43-276) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLK10
Gene ID: 5655
Uniprot ID: KLK10_HUMAN
Immunogen Region: 43-276
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 43-276 of human KLK10 (NP_665895.1).
Immunogen Sequence: LDPEAYGSPCARGSQPWQVS LFNGLSFHCAGVLVDQSWVL TAAHCGNKPLWARVGDDHLL LLQGEQLRRTTRSVVHPKYH QGSGPILPRRTDEHDLMLLK LARPVVLGPRVRALQLPYRC AQPGDQCQVAGWGTTAARRV KYNKGLTCSSITILSPKECE VFYPGVVTNNMICAGLDRGQ DPCQSDSGGPLVCDETLQGI LSWGVYPCGSAQHPAVYTQI CKYMSWINKVIRSN
Tissue Specificity Expressed in breast, ovary and prostate.
Function Has a tumor-suppressor role for NES1 in breast and prostate cancer.
Protein Name Kallikrein-10
Normal Epithelial Cell-Specific 1
Protease Serine-Like 1
Cellular Localisation Secreted
Alternative Antibody Names Anti-Kallikrein-10 antibody
Anti-Normal Epithelial Cell-Specific 1 antibody
Anti-Protease Serine-Like 1 antibody
Anti-KLK10 antibody
Anti-NES1 antibody
Anti-PRSSL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance