• Immunofluorescence analysis of MCF-7 cells using KMT5A/KMT5A/SETD8 antibody (STJ29444).
  • Western blot analysis of extracts of various cell lines, using KMT5A/KMT5A/SETD8 antibody (STJ29444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-KMT5A antibody (73-352) (STJ29444)

SKU:
STJ29444

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SETD8 (73-352) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KMT5A
Gene ID: 387893
Uniprot ID: KMT5A_HUMAN
Immunogen Region: 73-352
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 73-352 of human KMT5A/KMT5A/SETD8 (NP_065115.3).
Immunogen Sequence: ENSVTHHEVKCQGKPLAGIY RKREEKRNAGNAVRSAMKSE EQKIKDARKGPLVPFPNQKS EAAEPPKTPPSSCDSTNAAI AKQALKKPIKGKQAPRKKAQ GKTQQNRKLTDFYPVRRSSR KSKAELQSEERKRIDELIES GKEEGMKIDLIDGKGRGVIA TKQFSRGDFVVEYHGDLIEI TDAKKREALYAQDPSTGCYM YYFQYLSKTYCVDATRETNR LGRLINHSKCGNCQTKLHD
Post Translational Modifications Acetylated at Lys-162.does not affect methyltransferase activity. Deacetylated at Lys-162 possibly by SIRT2.does not change methyltransferase activity. Ubiquitinated and degraded by the DCX(DTL) complex.
Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Protein Name N-Lysine Methyltransferase Kmt5a
H4-K20-Hmtase Kmt5a
Histone-Lysine N-Methyltransferase Kmt5a
Lysine N-Methyltransferase 5a
Lysine-Specific Methylase 5a
Pr/Set Domain-Containing Protein 07
Pr-Set7
Pr/Set07
Set Domain-Containing Protein 8
Database Links Reactome: R-HSA-2299718
Reactome: R-HSA-3214841
Reactome: R-HSA-6804760
Cellular Localisation Nucleus
Chromosome
Specifically Localizes To Mitotic Chromosomes
Colocalized With Sirt2 At Mitotic Foci
Associates With Chromosomes During Mitosis
Association Is Increased In A H(2)O(2)-Induced Oxidative Stress-Dependent Manner
Associates With Silent Chromatin On Euchromatic Arms
Not Associated With Constitutive Heterochromatin
Alternative Antibody Names Anti-N-Lysine Methyltransferase Kmt5a antibody
Anti-H4-K20-Hmtase Kmt5a antibody
Anti-Histone-Lysine N-Methyltransferase Kmt5a antibody
Anti-Lysine N-Methyltransferase 5a antibody
Anti-Lysine-Specific Methylase 5a antibody
Anti-Pr/Set Domain-Containing Protein 07 antibody
Anti-Pr-Set7 antibody
Anti-Pr/Set07 antibody
Anti-Set Domain-Containing Protein 8 antibody
Anti-KMT5A antibody
Anti-PRSET7 antibody
Anti-SET07 antibody
Anti-SET8 antibody
Anti-SETD8 antibody

Information sourced from Uniprot.org

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