| Host: |
Mouse |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Krueppel-like factor 4 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
1E6 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KLF4 |
| Gene ID: |
9314 |
| Uniprot ID: |
KLF4_HUMAN |
| Specificity: |
GKLF Monoclonal Antibody detects endogenous levels of GKLF protein. |
| Immunogen: |
Purified recombinant fragment of human GKLF expressed in E. Coli. |
| Function | Transcription factor.can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. |
| Protein Name | Krueppel-Like Factor 4Epithelial Zinc Finger Protein EzfGut-Enriched Krueppel-Like Factor |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-422085Reactome: R-HSA-452723Reactome: R-HSA-9617828 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Krueppel-Like Factor 4 antibodyAnti-Epithelial Zinc Finger Protein Ezf antibodyAnti-Gut-Enriched Krueppel-Like Factor antibodyAnti-KLF4 antibodyAnti-EZF antibodyAnti-GKLF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and KLF4 knockout (KO) HeLa cells, using [KO Validated] KLF4 Rabbit polyclonal antibody (STJ11100061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89131/357580/STJ11100061_1__16470.1713121712.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and KLF4 knockout (KO) HeLa cells, using [KO Validated] KLF4 Rabbit polyclonal antibody (STJ11100061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
