Anti-KMT5C antibody (2-248 aa) (STJ11107082)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KMT5C (2-248 aa) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KMT5C |
| Gene ID | 84787 |
| Uniprot ID | KMT5C_HUMAN |
| Immunogen Region | 2-248 aa |
| Immunogen Sequence | GPDRVTARELCENDDLATSL VLDPYLGFRTHKMNVSPVPP LRRQQHLRSALETFLRQRDL EAAYRALTLGGWTARYFQSR GPRQEAALKTHVYRYLRAFL PESGFTILPCTRYSMETNGA KIVSTRAWKKNEKLELLVGC IAELREADEGLLRAGENDFS IMYSTRKRSAQLWLGPAAFI NHDCKPNCKFVPADGNAACV KVLRDIEPGDEVTCFYGEGF FGEKNEHCECHTCERKGEG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 2-248 of human KMT5C (NP_116090.2). |
Additional Info
| Function | Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. |
| Protein Name | Histone-Lysine N-Methyltransferase Kmt5cLysine N-Methyltransferase 5cLysine-Specific Methyltransferase 5cSuppressor Of Variegation 4-20 Homolog 2Su(Var4-20 Homolog 2Suv4-20h2Histone H4-N-Methyl-L-Lysine20 N-Methyltransferase Kmt5bHistone H4-Lysine20 N-Methyltransferase Kmt5b |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | NucleusChromosomeAssociated With Pericentric HeterochromatinCbx1 And Cbx5 Are Required For The Localization To Pericentric Heterochromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Kmt5c antibodyAnti-Lysine N-Methyltransferase 5c antibodyAnti-Lysine-Specific Methyltransferase 5c antibodyAnti-Suppressor Of Variegation 4-20 Homolog 2 antibodyAnti-Su(Var4-20 Homolog 2 antibodyAnti-Suv4-20h2 antibodyAnti-Histone H4-N-Methyl-L-Lysine20 N-Methyltransferase Kmt5b antibodyAnti-Histone H4-Lysine20 N-Methyltransferase Kmt5b antibodyAnti-KMT5C antibodyAnti-SUV420H2 antibodyAnti-PP7130 antibody |
Information sourced from Uniprot.org