• Immunofluorescence analysis of L929 cells using KLF8 Rabbit polyclonal antibody (STJ118751) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A431 cells using KLF8 Rabbit polyclonal antibody (STJ118751) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using KLF8 Rabbit polyclonal antibody (STJ118751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KLF8 antibody (210-280) (STJ118751)

SKU:
STJ118751

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLF8 (210-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF8
Gene ID: 11279
Uniprot ID: KLF8_HUMAN
Immunogen Region: 210-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-280 of human KLF8 (NP_009181.2).
Immunogen Sequence: GKNAGSVKVDPTSMSPLEIP SDSEESTIESGSSALQSLQG LQQEPAAMAQMQGEESLDLK RRRIHQCDFAG
Tissue Specificity Ubiquitous.
Post Translational Modifications Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.
Function Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
Protein Name Krueppel-Like Factor 8
Basic Krueppel-Like Factor 3
Zinc Finger Protein 741
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Krueppel-Like Factor 8 antibody
Anti-Basic Krueppel-Like Factor 3 antibody
Anti-Zinc Finger Protein 741 antibody
Anti-KLF8 antibody
Anti-BKLF3 antibody
Anti-ZNF741 antibody

Information sourced from Uniprot.org

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