• Western blot analysis of extracts of various cell lines, using KLHDC3 antibody (STJ115692) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of HeLa cells using KLHDC3 rabbit polyclonal antibody (STJ115692) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using KLHDC3 rabbit polyclonal antibody (STJ115692) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KLHDC3 antibody (1-382) (STJ115692)

SKU:
STJ115692

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLHDC3 (1-382) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLHDC3
Gene ID: 116138
Uniprot ID: KLDC3_HUMAN
Immunogen Region: 1-382
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human KLHDC3 (NP_476502.1).
Immunogen Sequence: MLRWTVHLEGGPRRVNHAAV AVGHRVYSFGGYCSGEDYET LRQIDVHIFNAVSLRWTKLP PVKSAIRGQAPVVPYMRYGH STVLIDDTVLLWGGRNDTEG ACNVLYAFDVNTHKWFTPRV SGTVPGARDGHSACVLGKIM YIFGGYEQQADCFSNDIHKL DTSTMTWTLICTKGSPARWR DFHSATMLGSHMYVFGGRAD RFGPFHSNNEIYCNRIRVFD TRTEAWLDCPPTPVLPEGR
Function Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(KLHDC3) complex specifically recognizes proteins with a glycine (Gly) at the C-terminus, leading to their ubiquitination and degradation: recognizes the C-terminal -Arg-(Xaa)n-Arg-Gly, -Arg-(Xaa)n-Lys-Gly, and -Arg-(Xaa)n-Gln-Gly degrons. The CRL2(KLHDC3) complex mediates ubiquitination and degradation of truncated SELENOV and SEPHS2 selenoproteins produced by failed UGA/Sec decoding, which end with a glycine. May be involved in meiotic recombination process.
Protein Name Kelch Domain-Containing Protein 3
Testis Intracellular Mediator Protein
Database Links Reactome: R-HSA-381038
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Kelch Domain-Containing Protein 3 antibody
Anti-Testis Intracellular Mediator Protein antibody
Anti-KLHDC3 antibody
Anti-PEAS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance