Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using MHC class I (HLA-A/B) Rabbit mAb (STJ11102369) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-HLA-A/HLA-B antibody (200-300) [S9MR] (STJ11102369)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunohistochemistry analysis of HLA-B in paraffin-embedded mouse spleen using HLA-B Rabbit polyclonal antibody (STJ24022) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HLA-B antibody (1-100) (STJ24022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using HLA-C antibody (STJ111053) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HLA-C antibody (25-308) (STJ111053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-HLA-D antibody [7.5.10.1] (STJ16100014)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF

Reactivity :

Human

£221.50

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-HLA-DMB antibody (40-100 aa) (STJ98988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various cells using HLA-DO Alpha Polyclonal Antibody

Anti-HLA-DOA antibody (71-120 aa) (STJ93542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Confocal imaging of paraffin-embedded Mouse spleen tissue using HLA-DPB1 Rabbit mAb (STJ11107363, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-HLA-DPB1 antibody (1-100 aa) [SRM] (STJ11107363)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of Raji cells using HLA-DPB1 Rabbit polyclonal antibody (STJ115276) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HLA-DPB1 antibody (40-100) (STJ115276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using HLA-DPB1 Rabbit polyclonal antibody (STJ119591) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HLA-DPB1 antibody (40-100) (STJ119591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

Anti-HLA-DQA1 antibody (21-70 aa) (STJ98824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of PC12 cells using HLA-DQB1/2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HLA-DQB1/2 antibody (131-180 aa) (STJ96857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Anti-HLA-DR antibody (100-200 aa) [SRM] (STJ11107240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-HLA-DRw52 antibody [7.3.19.1] (STJ16100015)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF

Reactivity :

Human

£221.50

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Western blot analysis of various cells using HLX1 Polyclonal Antibody diluted at 1：1000

Anti-HLX antibody (321-370 aa) (STJ93546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of MCF7 cells, using GPR109 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HM74 antibody (285-334 aa) (STJ93547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A-549 cells using HMG20A antibody (STJ29425).

Anti-HMG20A antibody (1-347) (STJ29425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using BRAF35 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-HMG20B antibody (1-50 aa) (STJ91880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGA1 antibody (STJ11102046) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-HMGA1 antibody (1-107) [S6MR] (STJ11102046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HMGA1 antibody (20-60 aa) (STJ196532)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1 Rabbit polyclonal antibody (STJ11105655) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGB1 antibody (1-215) (STJ11105655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using HMGB1 antibody (STJ11101678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HMGB1 antibody (100-200) [S8MR] (STJ11101678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, HMG-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HMGB1 antibody (N-Term) (STJ96867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-HMGB1 antibody [DPH11] (STJA0019032)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/FuncS/IF/WB

Reactivity :

Human/Mouse/Rat/Bovine/Dog/Horse/Pig

£378.50 - £1,632.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g HMGB2 Rabbit mAb (STJ11102444) , along with a Goat Anti-Rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of HMGB2 in representative gene loci (PTBP1) , as shown in figure.

Anti-HMGB2 antibody (1-100) [S4MR] (STJ11102444)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using HMGB2 Rabbit polyclonal antibody (STJ119486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMGB2 antibody (100-200) (STJ119486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using HMG-2 Polyclonal Antibody

Anti-HMGB2 antibody (131-180 aa) (STJ93552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Mouse liver tissue using HMGCR Rabbit polyclonal antibody (STJ11105656) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HMGCR antibody (200-300) (STJ11105656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using HMGCR Rabbit polyclonal antibody (STJ119212) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGCR antibody (351-440) (STJ119212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded Human liver tissue using HMGCR Rabbit polyclonal antibody (STJ115278) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HMGCR antibody (629-888) (STJ115278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using (STJ119211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HMGCR antibody (783-791) (STJ119211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Rat brain, using HMGCR rabbit polyclonal antibody (STJ24046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HMGCR antibody (783-791) (STJ24046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ71766 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-HMGCR/LDLCQ3 antibody (Internal) (STJ71766)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Immunofluorescence analysis of HeLa cells using HMGCS1 Rabbit polyclonal antibody (STJ24047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGCS1 antibody (241-520) (STJ24047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using HMGCS2 rabbit polyclonal antibody (STJ116457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMGCS2 antibody (269-508) (STJ116457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HMGN1 antibody (1-100 aa) (STJ11106363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using HMGN1 antibody (STJ27908) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HMGN1 antibody (1-100) (STJ27908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells, using HMG14 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HMGN1 antibody (10-59 aa) (STJ93549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using HMG17 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HMGN2 antibody (1-50 aa) (STJ93550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SMF Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HMGXB3 antibody (131-180 aa) (STJ95706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMMR antibody (1-300) (STJ114319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 293T lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-HMMR antibody (280-340 aa) (STJ98724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of Raw264. 7 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (STJ11101600) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HMOX1 antibody (1-265) [S0MR] (STJ11101600)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using Hmox1 Rabbit polyclonal antibody (STJ11105174) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-Hmox1 antibody (1-266) (STJ11105174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 (HO-1/HMOX1) antibody (STJ11104351) at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.

Anti-HMOX1 antibody (1-288) (STJ11104351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ116419) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HMOX1 antibody (1-288) (STJ116419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 Cell lysate using HO-1 mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HMOX1 antibody [Mix] (STJ196663)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various cells using HNF-4 Alpha/Gamma Polyclonal Antibody

Anti-HNF-4 Alpha/Gamma antibody (91-140 aa) (STJ93555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-HNF1A antibody (C-Term) (STJ73843)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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