• Immunofluorescence analysis of U-2 OS cells using EIF5B Rabbit polyclonal antibody (STJ117317) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using EIF5B Rabbit polyclonal antibody (STJ117317) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using EIF5B Rabbit polyclonal antibody (STJ117317) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using EIF5B Rabbit polyclonal antibody (STJ117317). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EIF5B antibody (1-273) (STJ117317)

SKU:
STJ117317

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF5B (1-273) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF5B
Gene ID: 9669
Uniprot ID: IF2P_HUMAN
Immunogen Region: 1-273
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3).
Immunogen Sequence: MGKKQKNKSEDSTKDDIDLD ALAAEIEGAGAAKEQEPQKS KGKKKKEKKKQDFDEDDILK ELEELSLEAQGIKADRETVA VKPTENNEEEFTSKDKKKKG QKGKKQSFDDNDSEELEDKD SKSKKTAKPKVEMYSGSDDD DDFNKLPKKAKGKAQKSNKK WDGSEEDEDNSKKIKERSRI NSSGESGDESDEFLQSRKGQ KKNQKNKPGPNIESGNEDDD ASFKIKTVAQKKAEKKERE
Post Translational Modifications (Microbial infection) Cleaved and inactivated by the protease 3C of poliovirus, Coxsackievirus B3 and Human rhinovirus 14, allowing the virus to shutoff the host cell translation.
Function Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit.
Protein Name Eukaryotic Translation Initiation Factor 5b
Eif-5b
Translation Initiation Factor If-2
Database Links Reactome: R-HSA-72706
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 5b antibody
Anti-Eif-5b antibody
Anti-Translation Initiation Factor If-2 antibody
Anti-EIF5B antibody
Anti-IF2 antibody
Anti-KIAA0741 antibody

Information sourced from Uniprot.org

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