Anti-EIF5 antibody (1-235) (STJ28666)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EIF5 (1-235) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF5 |
| Gene ID | 1983 |
| Uniprot ID | IF5_HUMAN |
| Immunogen Region | 1-235 |
| Immunogen Sequence | MSVNVNRSVSDQFYRYKMPR LIAKVEGKGNGIKTVIVNMV DVAKALNRPPTYPTKYFGCE LGAQTQFDVKNDRYIVNGSH EANKLQDMLDGFIKKFVLCP ECENPETDLHVNPKKQTIGN SCKACGYRGMLDTHHKLCTF ILKNPPENSDSGTGKKEKEK KNRKGKDKENGSVSSSETPP PPPPPNEINPPPHTMEEEED DDWGEDTTEEAQRRRMDEIS DHAKVLTLSDDLERT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2). |
Additional Info
| Function | Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC. When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP. Start codon recognition also induces a conformational change of the PIC to a closed state. This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC. Finally, EIF5 stabilizes the PIC in its closed conformation. |
| Protein Name | Eukaryotic Translation Initiation Factor 5Eif-5 |
| Database Links | Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 5 antibodyAnti-Eif-5 antibodyAnti-EIF5 antibody |
Information sourced from Uniprot.org