• Western blot analysis of extracts of Rat skeletal muscle, using EIF4B antibody (STJ115264) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of extracts of mouse kidney, using EIF4B antibody (STJ115264) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of A-549 using EIF4B antibody (STJ115264) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 using EIF4B antibody (STJ115264) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa using EIF4B antibody (STJ115264) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF4B antibody (352-611) (STJ115264)

SKU:
STJ115264

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF4B (352-611) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4B
Gene ID: 1975
Uniprot ID: IF4B_HUMAN
Immunogen Region: 352-611
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
Immunogen Sequence: SQSTRAASIFGGAKPVDTAA REREVEERLQKEQEKLQRQL DEPKLERRPRERHPSWRSEE TQERERSRTGSESSQTGTST TSSRNARRRESEKSLENETL NKEEDCHSPTSKPPKPDQPL KVMPAPPPKENAWVKRSSNP PARSQSSDTEQQSPTSGGGK VAPAQPSEEGPGRKDENKVD GMNAPKGQTGNSSRGPGDGG NRDHWKESDRKDGKKDQDSR SAPEPKKPEENPASKFSSA
Post Translational Modifications Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex. In response to mTORC1 activation, RPS6KA1-mediated phosphorylation at 'Ser-406' and 'Ser-422' stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function.
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Protein Name Eukaryotic Translation Initiation Factor 4b
Eif-4b
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-166208
Reactome: R-HSA-429947
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4b antibody
Anti-Eif-4b antibody
Anti-EIF4B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance