Immunocytochemistry Antibodies | St John's Laboratory

Immunofluorescence analysis of U-2 OS cells using PHKG2 Rabbit polyclonal antibody (STJ29476) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PHKG2 antibody (237-406) (STJ29476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-PHLDA1 antibody [R02-9F4] (STJA0034719)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of lysates from PC-3 cells using PHLDA2 rabbit polyclonal antibody (STJ28000) at 1:4000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:180s.

Anti-PHLDA2 antibody (1-152) (STJ28000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various lysates using PHLPP1 Rabbit pAb (STJ111739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-PHLPP1 antibody (1160-1428) (STJ111739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of L929 cells using PHLPP2 Rabbit polyclonal antibody (STJ11100175) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PHLPP2 antibody (1250-1323) (STJ11100175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-Phospho-ACC1/ACC2-S79 antibody [SRM5539] (STJ11107725)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Anti-Phospho-Acetyl CoA Carboxylase 1-S79-S79 antibody [SRM15134] (STJ11109864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-Phospho-ACK1 antibody (250-300) (STJ502083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$686.55

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Anti-Phospho-ACK1 antibody (250-300) {Biotin} (STJ502084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$761.07

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Anti-Phospho-ACK1 antibody (300-400) {FITC} (STJ502085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$761.07

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Anti-Phospho-ACTB-Tyr-53 antibody (STJA0005242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$504.39

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Anti-Phospho-Actin-Tyr-53 antibody (STJA0041066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$748.65

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Anti-Phospho-ACTR2-T237/T238 antibody (STJA0005267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$504.39

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Anti-Phospho-AGO2-Ser-387 antibody (STJA0005264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$504.39

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Anti-Phospho-AGO2-Tyr-393 antibody (STJA0005265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$504.39

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Anti-Phospho-AKT-Ser473 antibody [R06-7C5] (STJA0036228)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunohistochemistry analysis of paraffin-embedded Human brain using Phospho-Akt-Y315 Rabbit pAb (STJ11105776) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-Phospho-Akt-Y315 antibody (STJ11105776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$0.00

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Anti-Phospho-alpha Synuclein-Ser129 antibody [R01-5B1] (STJA0035883)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-alpha Synuclein-Ser129 antibody [R04-7E3] (STJA0035824)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-alpha Tubulin-Tyr272 antibody [R06-9Z1] (STJA0035837)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-AMPK alpha 1-Ser496 antibody [R01-3B7] (STJA0035051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-Amyloid Precursor Protein-Thr743 antibody [R07-4D0] (STJA0035052)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Anti-Phospho-Anti-Phosphoserine/threonine-pSer/Thr antibody (STJA0041345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Anti-Phosphoserine/threonine-pSer/Thr antibody [M380A/M380B] (STJA0041322)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Anti-Phosphotyrosine-p-Tyr antibody (STJA0041341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

$654.81

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Anti-Phospho-Anti-Phosphotyrosine-p-Tyr antibody [M375] (STJA0041321)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

$654.81

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Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (No. STJA0003582, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.

Anti-Phospho-AQP2-Ser 269 antibody (STJA0003582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC/IP

Reactivity :

Human/Mouse/Rat/Bovine/Canine

$674.13

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Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser261 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-AQP2-Ser261 antibody (STJA0003580)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC/IP

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates

$674.13

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Anti-Phospho-Argonaute 2-Ser-387 antibody (STJA0041078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Argonaute 2-Tyr-393 antibody (STJA0041079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Arp2-T237/T238 antibody (STJA0041071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$748.65

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Anti-Phospho-Asc-Tyr-144 antibody (STJA0041081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74 kDa ATF2 protein phosphorylated at Ser490 and Ser498.

Anti-Phospho-ATF2-Ser490/498 antibody (STJA0003583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

$674.13

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Anti-Phospho-ATF2-Thr71 antibody [R07-3E5] (STJA0034048)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Confocal imaging of paraffin-embedded Human colon tissue (untreated) and Human colon tissue (treated with Lambda pp) using Phospho-ATM-S1981 Rabbit mAb (STJ11102610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-ATM-S1981 antibody [S0MR] (STJ11102610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0005274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$504.39

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0041054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-ATM-Ser-794 antibody (STJA0005273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$504.39

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Anti-Phospho-ATM-Ser-794 antibody (STJA0041068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-ATM-Ser1981 antibody [R01-5I8] (STJA0035784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of lysates from 293T cells, using Phospho-ATR-S428 Rabbit pAb (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody (STJ114848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from Jurkat and 293 cells, using Phospho-Bcl-2-S70 Rabbit pAb (STJ29341) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-BCL2-S70 antibody (STJ29341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells using Phospho-Bcl-XL-S62 Rabbit pAb (STJ22039). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-BCL2L1-S62 antibody (STJ22039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-Beta-Catenin-Tyr-142 antibody (STJA0041137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Beta-Catenin-Tyr-489 antibody (STJA0041156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Beta-Catenin-Tyr-654 antibody (STJA0041163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Beta-Catenin-Tyr-86 antibody (STJA0041141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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