ELISA Antibodies | St John's Laboratory

STJ72199 (2µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-E2F4 antibody (Internal) (STJ72199)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Dog/Cow

£312.50

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Western blot analysis of lysates from A-549 cells, using E2F5 Rabbit polyclonal antibody (STJ118482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-E2F5 antibody (197-346) (STJ118482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-E2F5 antibody (5-55) (STJ500828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-E2F5 antibody (5-55) {Biotin} (STJ500829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-E2F5 antibody (5-55) {FITC} (STJ500830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Western blot analysis of HeLa cells using E2F-5 Polyclonal Antibody

Anti-E2F5 antibody (93-142 aa) (STJ92810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using E2F6 antibody (STJ11105714) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-E2F6 antibody (1-281) (STJ11105714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Mouse

£112.50 - £524.50

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Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (STJ116186) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-E2F6 antibody (1-281) (STJ116186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse

£112.50 - £524.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g E2F6 rabbit monoclonal antibody (STJ11101356) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of E2F6 in representative gene loci (BRCA1) , as shown in figure.

Anti-E2F6 antibody (110-281) [S6MR] (STJ11101356)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various cells using E2F-6 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F6 antibody (141-190 aa) (STJ92811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ71943 (1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-E2F6 antibody (C-Term) (STJ71943)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Cow

£312.50

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Western blot analysis of various lysates using E2F7 Rabbit polyclonal antibody (STJ117405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-E2F7 antibody (540-740) (STJ117405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-E2F7 antibody (560-640 aa) (STJ191781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ71884 (0. 3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-E2F7 antibody (Internal) (STJ71884)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Pig

£312.50

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Western blot analysis of various lysates using E2F8 Rabbit polyclonal antibody (STJ23459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-E2F8 antibody (788-867) (STJ23459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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STJ71792 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-E2F8 antibody (Internal) (STJ71792)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Cow

£312.50

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Anti-E2F8 antibody (STJ191922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-E3 antibody [RC10601] (STJ400338)

Clonality :

Monoclonal

Host :

Mouse, Human

Applications :

ELISA

Reactivity :

Estriol

£507.50 - £775.50

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Western blot analysis using E7 monoclonal antibody against recombinant protein.

Anti-E7 antibody (STJ98008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using E7 monoclonal antibody against truncated GST-E7 recombinant protein (1).

Anti-E7 antibody [3D6F1] (STJ98007)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemistry of rat tissue with Anti-EAAT1 primary antibody (STJ500831) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-EAAT1 antibody (480-543) (STJ500831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-EAAT1 antibody (480-543) {Biotin} (STJ500832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-EAAT1 antibody (480-543) {FITC} (STJ500833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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STJ72934 (0. 1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EAAT1/GLAST1 antibody (197-208) (STJ72934)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Rat

£312.50

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STJ72935 (2µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EAAT1/GLAST1 antibody (C-Term) (STJ72935)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of various lysates using EAF1 Rabbit polyclonal antibody (STJ119827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EAF1 antibody (50-120) (STJ119827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A-549 cells using EAF2 antibody (STJ29199).

Anti-EAF2 antibody (1-170) (STJ29199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from mouse kidney, using EARS2 Rabbit polyclonal antibody (STJ117158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-EARS2 antibody (42-220) (STJ117158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-Eat2A/Eat2B-Mouse antibody (C-Term) (STJ70937)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Mouse

£193.50

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Anti-EB2 antibody (1-50) (STJ506103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Zebra Fish

£427.50

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Anti-EB2 antibody (1-50) {Biotin} (STJ506104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Zebra Fish

£528.50

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Anti-EB2 antibody (1-50) {FITC} (STJ506105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Zebra Fish

£528.50

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Anti-EB2 antibody (150-210) (STJ506106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Zebra Fish

£427.50

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Anti-EB2 antibody (150-210) {Biotin} (STJ506107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Zebra Fish

£528.50

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Anti-EB2 antibody (150-210) {FITC} (STJ506108)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Zebra Fish

£528.50

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Confocal imaging of C2C12 cells using EB3/MAPRE3 Rabbit monoclonal antibody (STJ11103122, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-EB3/MAPRE3 antibody (182-281) [S2MR] (STJ11103122)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using EBAG9 antibody (STJ23462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-EBAG9 antibody (28-213) (STJ23462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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STJ70947 (0. 2µg/ml) staining of A431 lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EBAG9/RCAS1 antibody (C-Term) (STJ70947)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot analysis of various lysates, using EBF1 antibody (STJ114934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-EBF1 antibody (500-591) (STJ114934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from Mouse kidney, using EBF1 Rabbit pAb (STJ115908) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-EBF1 antibody (C-Term) (STJ115908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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STJ71185 (0. 3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EBF1/COE1 antibody (Internal) (STJ71185)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IP

Reactivity :

Human/Mouse/Rat

£312.50

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Anti-EBF2 antibody (STJA0004792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Western blot analysis of Rat thymus, using EBF3 Rabbit polyclonal antibody (STJ118418) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-EBF3 antibody (400-500) (STJ118418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Anti-EBF3 antibody (50-130 aa) (STJ191533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-EBF4 antibody (50-150 aa) (STJ11106192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-EBFP antibody [3A6] (STJ16100947)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP

Reactivity :

Human

£580.50

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Anti-EBFP antibody [7F10] (STJ16100948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP

Reactivity :

Human

£509.50

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Western blot analysis of HBE cells using Ebi3 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-EBI3 antibody (101-150 aa) (STJ97340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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