| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CRACR2A (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CRACR2A |
| Gene ID: |
84766 |
| Uniprot ID: |
EFC4B_HUMAN |
| Immunogen Region: |
1-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CRACR2A (NP_001138430.1). |
| Immunogen Sequence: |
MAAPDGRVVSRPQRLGQGSG QGPKGSGACLHPLDSLEQKE TQEQTSGQLVMLRKAQEFFQ TCDAEGKGFIARKDMQRLHK ELPLSLEELEDVFDALDADG NGYLTPQEFTTGFSHFFFSQ NNPSQEDAGEQVAQRHEEKV YLSRGDEDLGDMGEDEEAQF RMLMDRLGAQKVLEDESDVK QLWLQLKKEEPHLLSNFEDF |
| Tissue Specificity | Isoform 1: Expressed in the Jurkat T-cell line. Isoform 2: Expressed in endothelial cells. Expressed in Weibel-Palade bodies (which are P-selectin/SELP negative) in endothelial cells. Expressed in the Jurkat T-cell line. |
| Function | Isoform 1: Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex. Isoform 2: Rab GTPase that mediates the trafficking of Weibel-Palade bodies (WPBs) to microtubule organizing center (MTOC) in endothelial cells in response to acute inflammatory stimuli. During histamine (but not thrombin) stimulation of endothelial cells, the dynein-bound form induces retrograde transport of a subset of WPBs along microtubules to the MTOC in a Ca(2+)-independent manner and its GTPase activity is essential for this function. Ca(2+)-regulated dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules and regulates endosomal trafficking of CD47. Acts as an intracellular signaling module bridging two important T-cell receptor (TCR) signaling pathways, Ca(2+)-NFAT and JNK, to affect T-cell activation. In resting T-cells, is predominantly localized near TGN network in a GTP-bound form, upon TCR stimulation, localizes at the immunological synapse via interaction with VAV1 to activate downstream Ca(2+)-NFAT and JNK signaling pathways. Plays a role in T-helper 1 (Th1) cell differentiation and T-helper 17 (Th17) cell effector function. Plays a role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. |
| Protein Name | Ef-Hand Calcium-Binding Domain-Containing Protein 4bCalcium Release-Activated Calcium Channel Regulator 2aCrac Channel Regulator 2aCalcium Release-Activated Channel Regulator 2aRas-Related Protein Rab-46 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 2: CytoplasmCytoplasmCytoskeletonMicrotubule Organizing CenterCell MembraneGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneVesicleT-Cell Activation-Induced Elevation Of Intracellular Of Ca(2+) Stimulates Its Transport Toward The Microtubule Organizing Center (Mtoc)Histamine Stimulation Induces A Trafficking To The Mtoc In A Gtp-Binding-Dependent But Ca(2+)-Binding-Independent MannerLocalizes To Golgi Membrane In Resting T-Cells And Upon Its Interaction With Vav1Is Translocated From The Golgi Membrane To The Immunological Synapse Via Subsynaptic VesiclesIts Localization In The Golgi Membrane Requires Isoprenylation And Gtp-Binding |
| Alternative Antibody Names | Anti-Ef-Hand Calcium-Binding Domain-Containing Protein 4b antibodyAnti-Calcium Release-Activated Calcium Channel Regulator 2a antibodyAnti-Crac Channel Regulator 2a antibodyAnti-Calcium Release-Activated Channel Regulator 2a antibodyAnti-Ras-Related Protein Rab-46 antibodyAnti-CRACR2A antibodyAnti-EFCAB4B antibodyAnti-RAB46 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
