ELISA Antibodies | St John's Laboratory

Western blot analysis of extracts of HepG2 cells, using PD-L1/CD274 antibody (STJ11104347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (190-290) (STJ11104347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ22985) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD274 antibody (190-290) (STJ22985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry of baboon spleen with Anti-CD274 primary antibody (STJ502399) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD274 antibody (80-130) (STJ502399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$577.13

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Anti-CD274 antibody (80-130) {Biotin} (STJ502400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Anti-CD274 antibody (80-130) {FITC} (STJ502401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274/CD274 Rabbit monoclonal antibody (STJ11103966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (ECD) [S3966RM] (STJ11103966)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$174.83 - $775.58

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Anti-CD274 antibody [SMM] (STJ11106420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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STJ70646 (0. 01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD274/PD-L1 antibody (C-Term) (STJ70646)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

$421.88

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Biotinylated STJ70646 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-CD274/PD-L1, Biotinylated antibody (C-Term) {Biotin} (STJ73200)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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Western blot analysis of Mouse spleen, using CD275 Rabbit polyclonal antibody (STJ11105078) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD275 antibody (47-270 aa) (STJ11105078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Western blot analysis of 293 cells using CD276 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD276 antibody (271-320 aa) (STJ96650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of CD276 in paraffin-embedded human thyroid cancer tissue using CD276 Rabbit polyclonal antibody (STJ11105431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CD276 antibody (29-245) (STJ11105431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various lysates, using CD276/B7-H3 rabbit polyclonal antibody (STJ119347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : RajiExposure time: 180s.

Anti-CD276 antibody (29-245) (STJ119347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis using CD276 monoclonal antibody against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CD276 antibody [6A1] (STJ97917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of various lysates, using CD276/B7-H3 Rabbit monoclonal antibody (STJ11105227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : RajiExposure time: 90s.

Anti-CD276/B7-H3 antibody [S5227RM] (STJ11105227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis of K562 cells using CD28 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD28 antibody (11-60 aa) (STJ96609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Flow cytometric analysis of CD28 Rabbit monoclonal antibody Cocotail (10 Mu g/mL) in Jurkat cells (Orange) compare to Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) Isotype control (Blue) and non-staining control (Red).

Anti-CD28 antibody (Full Length) [S5MR] (STJ11103275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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STJ72503 (0. 3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD28 antibody (Internal) (STJ72503)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

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STJ72504 (0. 3µg/ml) staining of Mouse Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Cd28-Mouse antibody (Internal) (STJ72504)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

$421.88

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Anti-CD298/ATP1B3 antibody (180-279 aa) [SRM] (STJ11106584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD299/DC-SIGNR using CD299/DC-SIGNR Rabbit mAb (STJ11107384) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 180s.

Anti-CD299/DC-SIGNR antibody (71-399 aa) [SRM] (STJ11107384)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

$174.83 - $775.58

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Anti-CD2AP antibody (320-400 aa) (STJ191673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using CD2AP Rabbit polyclonal antibody (STJ110098) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Cd2ap antibody (360-639) (STJ110098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-CD2AP antibody (C-Term) (STJ118925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from wild type (WT) and CD2BP2 knockout (KO) 293T cells, using [KO Validated] CD2BP2 Rabbit polyclonal antibody (STJ11100767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD2BP2 antibody (1-341) (STJ11100767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of various lysates using CD2BP2 Rabbit polyclonal antibody (STJ118222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD2BP2 antibody (1-341) (STJ118222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various cells using CD2BP2 Polyclonal Antibody diluted at 1：1000

Anti-CD2BP2 antibody (101-150 aa) (STJ92111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

$64.80 - $340.20

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STJ70564 (0. 3µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD2BP2 antibody (N-Term) (STJ70564)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

$421.88

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STJ73100 (0. 1µg/ml) staining of Jurkat (A) and (0. 3ug/ml) MOLT4 (B) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD3-ZETA/CD247 antibody (Internal) (STJ73100)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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Western blot analysis of lysates from A-549 cells, using CD30 Rabbit mAb (STJ11103680) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-CD30 antibody (496-595) [S0MR] (STJ11103680)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

$174.83 - $775.58

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-CD300A antibody (141-190 aa) (STJ98999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of THP-1, using CD300A antibody (STJ112046) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CD300A antibody (18-180) (STJ112046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of rat kidney cells using CD300c Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-CD300C antibody (131-180 aa) (STJ97314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using CD300C antibody (STJ112452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CD300C antibody (21-183) (STJ112452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-CD300E antibody (18-118 aa) (STJ11106416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Anti-CD300E antibody (31-80 aa) (STJ98749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using CD300LB Rabbit polyclonal antibody (STJ11103366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD300LB antibody (1-201) (STJ11103366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200

Anti-CD300LB antibody (61-110 aa) (STJ98765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of NIH-3T3 cells using CD300d Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD300LD antibody (61-110 aa) (STJ97331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using CD300LF Rabbit polyclonal antibody (STJ115771) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD300LF antibody (23-150) (STJ115771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using CD300LF Rabbit polyclonal antibody (STJ115772) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD300LF antibody (23-150) (STJ115772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunohistochemistry with Anti-CD300LF Antibody (STJ500460) at 1:100 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-CD300LF antibody (230-290) (STJ500460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

$577.13

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Anti-CD300LF antibody (230-290) {Biotin} (STJ500461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

$713.48

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Anti-CD300LF antibody (230-290) {FITC} (STJ500462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$713.48

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Western blot analysis of HeLa, PC12 cells using CD300f Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD300LF antibody (41-90 aa) (STJ96840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-CD300LG antibody (1-332) (STJ11100499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Western blot analysis of L929 cells using CD300g Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD300LG antibody (41-90 aa) (STJ96637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using CD302 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CD302 antibody (51-100 aa) (STJ92666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options