• K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Topoisomerase IIα (ABT272) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 174kDaObserved band size: 174kDa
  • Human lung squamous cell carcinoma tissue was stained with Anti-Topoisomerase IIα (ABT272) Antibody
  • Human lymphoma tissue was stained with Anti-Topoisomerase IIα (ABT272) Antibody
  • Human seminoma tissue was stained with Anti-Topoisomerase IIα (ABT272) Antibody
  • Human testis tissue was stained with Anti-Topoisomerase IIα (ABT272) Antibody
  • Human tonsil tissue was stained with Anti-Topoisomerase IIα (ABT272) Antibody
  • Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Vimentin (STJ197198), Anti-p120 (STJ190083), Anti-Topoisomerase IIα (STJ197194). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit. The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (STJ197198 1:200), Anti-p120 (STJ190083 1:200), Anti-Topoisomerase IIα (STJ197194 1:200). Each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
  • Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Vimentin (STJ197198), Anti-p120 (STJ190083), Anti-Topoisomerase IIα (STJ197194). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit. The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (STJ197198 1:200), Anti-p120 (STJ190083 1:200), Anti-Topoisomerase IIα (STJ197194 1:200). ; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).

Anti-TOP2A antibody (1400-1531 aa) [ABT272] (STJ197194)

SKU:
STJ197194

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-DNA topoisomerase 2-alpha (1400-1531 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT272
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOP2A
Gene ID: 7153
Uniprot ID: TOP2A_HUMAN
Immunogen Region: 1400-1531 aa
Specificity: The antibody can specifically recognize human Topoisomerase II Alpha protein.
Immunogen: Synthesized peptide derived from the human Topoisomerase II Alpha at the amino acid range 1400-1531
Post Translational Modifications Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated.
Function Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation.
Protein Name Dna Topoisomerase 2-Alpha
Dna Topoisomerase Ii - Alpha Isozyme
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-4615885
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleolus
Alternative Antibody Names Anti-Dna Topoisomerase 2-Alpha antibody
Anti-Dna Topoisomerase Ii - Alpha Isozyme antibody
Anti-TOP2A antibody
Anti-TOP2 antibody

Information sourced from Uniprot.org

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