GST-Tag Antibodies

Glutathione S-transferase (GST) is a 26 kDa enzyme, originally the Schistosoma japonicum isozyme Sj26, fused to the N-terminus of a target protein in the pGEX expression vector system to allow single-step affinity purification on immobilised glutathione.¹ Because the tag is large relative to smaller peptide tags such as Myc or FLAG, it is usually removed by protease cleavage after purification, most often using a thrombin recognition site engineered between the tag and the target protein.

The tag can be fused to either terminus of a protein, and beyond purification, GST fusions are also used as a solubility chaperone that helps fusion partners fold and remain soluble rather than forming inclusion bodies. Our range covers monoclonal, polyclonal and goat anti-GST formats.