Avi-Tag Antibodies
Detection of Avi-Tag fusion proteins biotinylated by the E. coli ligase BirAThe Avi-Tag is a 15-amino acid peptide (GLNDIFEAQKIEWHE) recognised by the bacterial biotin ligase BirA, which attaches a single biotin molecule to the lysine residue within the sequence.1 This gives site-specific biotinylation at one defined position, rather than the random labelling of surface lysines produced by chemical biotinylation methods.2
Because only that one internal lysine is modified, the rest of the fused protein is left unchanged. The tag can be added at the N-terminus, C-terminus or in an exposed loop during construct design, and is enzymatically biotinylated either in cells co-expressing BirA or in vitro using purified BirA.2
Anti-Avi-Tag Antibody
Our current Avi-Tag antibody for detecting biotinylated Avi-Tag fusion proteins by Western Blot.
Looking for a Different Tag?
We supply antibodies against Avi-Tag, His-Tag, FLAG/DDDDK-Tag, HA-Tag, Myc-Tag, GST-Tag, V5-Tag, T7-Tag, Strep-Tag, S-Tag, GFP-Tag and mCherry-Tag fusion proteins.
References
1. Fairhead M, Howarth M. Site-specific biotinylation of purified proteins using BirA. Methods Mol Biol. 2015. Link
2. Zhou Z, et al. Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation. Protein Expr Purif. 2012. Link
For Research Use Only. Not for use in diagnostic or therapeutic procedures.