| Host: | Mouse |
| Applications: | IHC/WB/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-DNA topoisomerase 2-alpha (1400-1531 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | ABT272 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: | IHC-P 1:100-500WB 1:200-1000IF 1:100-500 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TOP2A |
| Gene ID: | 7153 |
| Uniprot ID: | TOP2A_HUMAN |
| Immunogen Region: | 1400-1531 aa |
| Specificity: | The antibody can specifically recognize human Topoisomerase II Alpha protein. |
| Immunogen: | Synthesized peptide derived from the human Topoisomerase II Alpha at the amino acid range 1400-1531 |
| Post Translational Modifications | Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. |
| Protein Name | Dna Topoisomerase 2-AlphaDna Topoisomerase Ii - Alpha Isozyme |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-4615885 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmNucleolus |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Alpha antibodyAnti-Dna Topoisomerase Ii - Alpha Isozyme antibodyAnti-TOP2A antibodyAnti-TOP2 antibody |
Information sourced from Uniprot.org