Knockout-validated primary antibodies

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Knockout validated antibodies

Different validation methods have unique strengths and applications, but there will always be a margin of error about the exact specifications and uses of an antibody. For this reason, knockout validation is the most accepted and used as a standard throughout the life sciences research areas.
 
Essentially, this process confirms the specificity of an antibody by testing it either on a cell line or on a knockout sample, on which the protein of interest isn't expressed alongside a normal cell line. The target protein is missing from the cell line or knockout sample because the gene responsible for encoding this protein is not present and “knocked out” via disruption or replacement of the DNA. After the experiment is over, the results are compared and if there is no detection in the KO sample or cell line, but specific detection present in the normal cell line, the antibody can be categorised as specific.

 

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