![Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93441/374525/STJ114319_1__43866.1713141042.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and CD168/RHAMM knockout (KO) HeLa cells, using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93441/374526/STJ114319_2__27048.1713141042.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD168/RHAMM knockout (KO) HeLa cells, using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93441/374527/STJ114319_3__59212.1713141043.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/93441/374525/STJ114319_1__43866.1713141042.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-HMMR antibody (1-300) (STJ114319)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HMMR (1-300) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMMR |
| Gene ID | 3161 |
| Uniprot ID | HMMR_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MSFPKAPLKRFNDPSGCAPS PGAYDVKTLEVLKGPVSFQK SQRFKQQKESKQNLNVDKDT TLPASARKVKSSESKKESQK NDKDLKILEKEIRVLLQERG AQDRRIQDLETELEKMEARL NAALREKTSLSANNATLEKQ LIELTRTNELLKSKFSENGN QKNLRILSLELMKLRNKRET KMRGMMAKQEGMEMKLQVTQ RSLEESQGKIAQLEGKLVSI EKEKIDEKSETEKLLEYIE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1). |
Additional Info
| Tissue Specificity | Expressed in testis. Expressed in the breast. |
| Function | Receptor for hyaluronic acid (HA). Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis. |
| Protein Name | Hyaluronan Mediated Motility ReceptorIntracellular Hyaluronic Acid-Binding ProteinReceptor For Hyaluronan-Mediated MotilityCd Antigen Cd168 |
| Database Links | Reactome: R-HSA-2160916Reactome: R-HSA-8854518 |
| Cellular Localisation | Cell SurfaceCytoplasmCytoskeletonSpindle |
| Alternative Antibody Names | Anti-Hyaluronan Mediated Motility Receptor antibodyAnti-Intracellular Hyaluronic Acid-Binding Protein antibodyAnti-Receptor For Hyaluronan-Mediated Motility antibodyAnti-Cd Antigen Cd168 antibodyAnti-HMMR antibodyAnti-IHABP antibodyAnti-RHAMM antibody |
Information sourced from Uniprot.org