• Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and CD168/RHAMM knockout (KO) HeLa cells, using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-HMMR antibody (1-300) (STJ114319)

SKU:
STJ114319

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMMR (1-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMMR
Gene ID: 3161
Uniprot ID: HMMR_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1).
Immunogen Sequence: MSFPKAPLKRFNDPSGCAPS PGAYDVKTLEVLKGPVSFQK SQRFKQQKESKQNLNVDKDT TLPASARKVKSSESKKESQK NDKDLKILEKEIRVLLQERG AQDRRIQDLETELEKMEARL NAALREKTSLSANNATLEKQ LIELTRTNELLKSKFSENGN QKNLRILSLELMKLRNKRET KMRGMMAKQEGMEMKLQVTQ RSLEESQGKIAQLEGKLVSI EKEKIDEKSETEKLLEYIE
Tissue Specificity Expressed in testis. Expressed in the breast.
Function Receptor for hyaluronic acid (HA). Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis.
Protein Name Hyaluronan Mediated Motility Receptor
Intracellular Hyaluronic Acid-Binding Protein
Receptor For Hyaluronan-Mediated Motility
Cd Antigen Cd168
Database Links Reactome: R-HSA-2160916
Reactome: R-HSA-8854518
Cellular Localisation Cell Surface
Cytoplasm
Cytoskeleton
Spindle
Alternative Antibody Names Anti-Hyaluronan Mediated Motility Receptor antibody
Anti-Intracellular Hyaluronic Acid-Binding Protein antibody
Anti-Receptor For Hyaluronan-Mediated Motility antibody
Anti-Cd Antigen Cd168 antibody
Anti-HMMR antibody
Anti-IHABP antibody
Anti-RHAMM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance