![Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93441/374525/STJ114319_1__43866.1713141042.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and CD168/RHAMM knockout (KO) HeLa cells, using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93441/374526/STJ114319_2__27048.1713141042.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD168/RHAMM knockout (KO) HeLa cells, using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93441/374527/STJ114319_3__59212.1713141043.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/93441/374525/STJ114319_1__43866.1713141042.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HMMR |
| Gene ID: | 3161 |
| Uniprot ID: | HMMR_HUMAN |
| Immunogen Region: | 1-300 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1). |
| Immunogen Sequence: | MSFPKAPLKRFNDPSGCAPS PGAYDVKTLEVLKGPVSFQK SQRFKQQKESKQNLNVDKDT TLPASARKVKSSESKKESQK NDKDLKILEKEIRVLLQERG AQDRRIQDLETELEKMEARL NAALREKTSLSANNATLEKQ LIELTRTNELLKSKFSENGN QKNLRILSLELMKLRNKRET KMRGMMAKQEGMEMKLQVTQ RSLEESQGKIAQLEGKLVSI EKEKIDEKSETEKLLEYIE |
| Tissue Specificity | Expressed in testis. Expressed in the breast. |
| Function | Receptor for hyaluronic acid (HA). Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis. |
| Protein Name | Hyaluronan Mediated Motility Receptor Intracellular Hyaluronic Acid-Binding Protein Receptor For Hyaluronan-Mediated Motility Cd Antigen Cd168 |
| Database Links | Reactome: R-HSA-2160916 Reactome: R-HSA-8854518 |
| Cellular Localisation | Cell Surface Cytoplasm Cytoskeleton Spindle |
| Alternative Antibody Names | Anti-Hyaluronan Mediated Motility Receptor antibody Anti-Intracellular Hyaluronic Acid-Binding Protein antibody Anti-Receptor For Hyaluronan-Mediated Motility antibody Anti-Cd Antigen Cd168 antibody Anti-HMMR antibody Anti-IHABP antibody Anti-RHAMM antibody |
Information sourced from Uniprot.org