St John's Laboratory Ltd

Western blot analysis of A-549, using Neuropilin-1 (NRP1) antibody (STJ11104753) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:200000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-NRP1 antibody (22-644) [S4753RM] (STJ11104753)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot Neuropilin-1 detection in recombinant protein and PC3 cells STJA0041292

Anti-Neuropilin-1 antibody (a1 CUB Domain) [M534] (STJA0041292)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£542.50

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Immunofluorescence analysis of SH-SY5Y cells using Neuropilin-1 (NRP1) Rabbit polyclonal antibody (STJ119109) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NRP1 antibody (901-923) (STJ119109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Neuropilin 1 antibody western blot A549 mouse rat heart STJA0035843

Anti-Neuropilin 1 antibody [R03-7F2] (STJA0035843)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using Neuropilin-1 (NRP1) Rabbit monoclonal antibody (STJ11101624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NRP1 antibody (824-923) [S4MR] (STJ11101624)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using Neuropilin-1 Polyclonal Antibody

Anti-NRP1 antibody (476-525) (STJ94438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot COS-7 cells probed with anti-Neuropilin-1 antibody HA-tagged expression STJA0041294

Anti-Neuropilin-1 antibody (a1 CUB Domain) (STJA0041294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£542.50

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C-Myc Positive Control peptide (STJ503915)

Applications :

WB

£308.50

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Western blot analysis of lysates from HepG2 cells, using PKD1/PKC mu (Phospho-Tyr463) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKD1-Tyr463 antibody (429-478 aa) (STJ90881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, PKD1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PRKD1 antibody (171-220 aa) (STJ95136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using PKC alpha Rabbit monoclonal antibody (STJ11105041) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKCA antibody (200-300 aa) [S5041RM] (STJ11105041)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-PKC alpha antibody [SRM5132] (STJ11106955)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-Recombinant-PRKCA antibody [RM1Q36] (STJA0024020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Phospho-PKC Alpha-S657/Y658 antibody (STJA0041328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IP

Reactivity :

Human/Rabbit/Rat

£542.50

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Anti-MYC antibody (400-454) (STJ500592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Cat/Human/Monkey

£400.50

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Anti-MYC antibody (200-250) {Biotin} (STJ500593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Cat/Human/Monkey

£497.50

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Anti-MYC antibody (200-250) {FITC} (STJ500594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Cat/Human/Monkey

£497.50

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GPR56 Positive Control peptide (STJ504207)

Applications :

WB

£308.50

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Anti-Pan-PKC antibody [SM5008] (STJA0005008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Anti-Recombinant-PRKD1 antibody [RM2G06] (STJA0025574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Anti-Phospho-PKC alpha-Ser657 antibody [R05-8I3] (STJA0035085)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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Phospho-PKC alpha antibody western blot Jurkat lysate STJA0035672

Anti-Phospho-PKC alpha-Thr638 antibody [R84-8J-5] (STJA0035672)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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GPR56 Blocking Peptide peptide (STJ504157)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Anti-MYC antibody (400-454) {Biotin} (STJ500595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Cat/Human/Monkey

£497.50

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C-Myc Blocking Peptide peptide (STJ503865)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PKC alpha antibody (STJ112958). Western blot was performed from the immunoprecipitate using PKC alpha antibody (STJ112958) at a dilution of 1:1000.

Anti-PRKCA antibody (573-672) [S8MR] (STJ112958)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of 3T3 cells using Phospho-PKC Alpha (T638) Polyclonal Antibody

Anti-Phospho-PRKCA-Thr638 antibody (606-655 aa) (STJ90863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells using PKD1/2/3/PKC mu Antibody. The lane on the right is blocked STJ95137

Anti-PKD1/2/3 antibody (706-755 aa) (STJ95137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with STJ73233 (0. 1ug/ml) in top panel, probed with NA NA NA anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA.

Anti-PRKD1 antibody (383-395) (STJ73233)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB-Trf/IP

Reactivity :

Human

£312.50

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Rat PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017883)

Applications :

ELISA

Reactivity :

Rat

£453.50 - £631.50

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Anti-Recombinant-Phospho-PRKCA-Ser660 antibody [RM1Q35] (STJA0024019)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Anti-Recombinant-PRKD1 antibody [RM2G07] (STJA0025575)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Human PRKCA protein (Recombinant) (N-His) (STJP010877)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-Phospho-PKC alpha-Thr638 antibody [R09-7C7] (STJA0033921)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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Western Blot analysis of A431 cells using Phospho-PKD1 (S910) Polyclonal Antibody STJ90492

Anti-Phospho-PRKD1-Ser910 antibody (863-912 aa) (STJ90492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-PKC α (Y657) Polyclonal Antibody diluted at 1:1000 STJ90499

Anti-Phospho-PRKCA-Tyr657 antibody (623-672 aa) (STJ90499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with STJ73232 (0. 1ug/ml) in top panel, probed with NA NA NA anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA.

Anti-PRKD1 antibody (233-246) (STJ73232)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB-Trf/IP/IHC

Reactivity :

Human/Mouse/Rat/Pig

£312.50

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Human PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit (STJE0011495)

Applications :

ELISA

Reactivity :

Human

£404.50 - £562.50

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Anti-MYC antibody (400-454) {FITC} (STJ500596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Cat/Human/Monkey

£497.50

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Western blot analysis of lysates from C2C12 cells, using Phospho-PKC alpha-T638 Rabbit mAb (STJ11102617) at 1:1000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PRKCA-T638 antibody [S7MR] (STJ11102617)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western blot analysis of various lysates, using PKC alpha Rabbit polyclonal antibody (STJ115305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRKCA antibody (573-672) (STJ115305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon using PKC alpha antibody (STJ25125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PRKCA antibody (573-672) (STJ25125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of NIH-3T3 cells using PKC Alpha Polyclonal Antibody

Anti-PRKCA antibody (606-655 aa) (STJ95119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Rat PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit (STJE0011497)

Applications :

ELISA

Reactivity :

Rat

£432.50 - £602.50

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Human PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017881)

Applications :

ELISA

Reactivity :

Human

£423.50 - £587.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-PKC (PTR1190) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 70kDaObserved band size: 80kDa

Anti-PRKCA antibody (450-550) [PTR1190] (STJA0006229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit/Bovine

£49.00 - £261.00

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Anti-Recombinant-Phospho-PRKCA/PKC alpha-Ser657 antibody [RM1Q33] (STJA0024017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-PRKCA/PKC alpha-Thr638 antibody [RM1Q34] (STJA0024018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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