St John's Laboratory Ltd

Western blot analysis of lysates from wild type (WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (STJ119973) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-CDK2 antibody (200-298) (STJ119973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human colon using PKC alpha antibody (STJ25125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PRKCA antibody (573-672) (STJ25125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using PP2A Catalytic Alpha antibody (STJ28785) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PPP2CA antibody (1-309) (STJ28785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using ARK-2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-AURKB antibody (198-247 aa) (STJ91692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using AurB/C Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-AurB/C antibody (201-250 aa) (STJ91786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of NIH-3T3 cells using PKC Alpha Polyclonal Antibody

Anti-PRKCA antibody (606-655 aa) (STJ95119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ73294 (1µg/ml) staining of Thymus (A) , Lymph Node (B) , Tonsil (C) , Spleen (D) and Testis (E) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Aurora Kinase B antibody (Internal) (STJ73294)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ73296 (1µg/ml) staining HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-KAP1 antibody (Internal) (STJ73296)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot with Anti-MAPK11 Antibody (STJ501684) of 1) Rat cortex, 2) Mouse cerebellum, 3) Mouse lung, 4) HEK cells. Antibody is at 1:500 dilution in buffer.

Anti-MAPK11 antibody (300-350) (STJ501684)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-MAPK11 antibody (300-350) {Biotin} (STJ501685)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-SGK1 antibody (40-90) {FITC} (STJ502980)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£497.50

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Rat PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit (STJE0011497)

Applications :

ELISA

Reactivity :

Rat

£432.50 - £602.50

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Human PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017881)

Applications :

ELISA

Reactivity :

Human

£423.50 - £587.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-PKC (PTR1190) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 70kDaObserved band size: 80kDa

Anti-PRKCA antibody (450-550) [PTR1190] (STJA0006229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit/Bovine

£49.00 - £261.00

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Anti-Recombinant-Phospho-PRKCA/PKC alpha-Ser657 antibody [RM1Q33] (STJA0024017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-PRKCA/PKC alpha-Thr638 antibody [RM1Q34] (STJA0024018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-PRKCA antibody [RM1B40] (STJA0026810)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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SDS-PAGE gel of recombinant human KAP1 protein 3µg under reducing conditions Coomassie blue stain

Human KAP1 protein (Recombinant) (His-Tag) (STJP018428)

Host :

E.coli

Applications :

SDS-PAGE/Denatured

£134.50 - £738.50

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Anti-PKC Alpha antibody (Central Region) [M237] (STJA0041317)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£579.50

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SGK1 antibody immunohistochemistry human brain tissue STJA0032892

Anti-SGK1 antibody [6E4-8G5-6D10] (STJA0032892)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Phospho-CDK2 Tyr15 antibody western blot C6 UV lysates STJA0033906

Anti-Phospho-CDK2-Tyr15 antibody [R05-2C4] (STJA0033906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Hamster/Rat

£133.50 - £281.50

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Phospho-CDK2 Thr14 antibody western blot HeLa A549 HL-60 U2OS STJA0035056

Anti-Phospho-CDK2-Thr14 antibody [R06-4C1] (STJA0035056)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Parkin Positive Control peptide (STJ504572)

Applications :

WB

£308.50

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WB analysis phosphorylated parkin protein at Ser378 in HEK293 cells STJA0003743

Anti-Phospho-PARK2-Ser378 antibody (STJA0003743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£488.50

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Western blot analysis of various lysates using Aurora B Mouse mAb (STJ11107245) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-Aurora B antibody (1-100 aa) [SMM5339] (STJ11107245)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SDS-PAGE gel of recombinant human Parkin protein 3μg under reducing conditions Coomassie blue stain

Human Parkin protein (Recombinant) (His-Tag) (STJP018617)

Host :

E.coli

Applications :

SDS-PAGE/Denatured

£134.50 - £738.50

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Anti-Aurora B antibody (STJA0037828)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£187.50 - £281.50

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Anti-AURKB antibody (300-344) (STJ500192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human

£400.50

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Western blot with Anti-PRKN Antibody (STJ502119) on SKNMC cell at 1:500 dilution in buffer.

Anti-PRKN antibody (390-440) (STJ502119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£400.50

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Anti-PRKN antibody (390-440) {Biotin} (STJ502120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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Western blot phosphorylated parkin protein at Ser101 in HEK293 cells STJA0003742

Anti-Phospho-PARK2-Ser101 antibody (STJA0003742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£488.50

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Anti-Parkin antibody (C-Term) (STJ13100219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Anti-Parkinson disease protein 2 antibody (NSLIKELHHFRILGEEQ) (STJA0040869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/WB

Reactivity :

Human/Rat

£528.50

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Confocal imaging of HeLa cells using Aurora B Rabbit monoclonal antibody (STJ11101686, dilution 1:800) (Red). The cells were counterstained with Vimentin Rabbit monoclonal antibody (A19607, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-AURKB antibody (1-100) [S6MR] (STJ11101686)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-AURKB antibody (300-344) {FITC} (STJ500194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£497.50

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Anti-SIRT7 antibody (350-400) {Biotin} (STJ503045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot of recombinant human Aurora kinase B with anti-human Aurora B antibody

Anti-Aurora B antibody (1-344aa) [AT2B1] (STJA0041592)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-Parkinson disease protein 2 antibody (RILGEEQYNRYQQYGAEEC) (STJA0040868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC-F/WB

Reactivity :

Guinea Pig/Human/Rat

£528.50

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Western blot analysis of 293T AD293 Hela using p-Parkin (S131) antibody. Antibody was diluted at 1:500

Anti-Phospho-PRKN-Ser131 antibody (101-150 aa) (STJ91122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Parkin Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PRKN antibody (1-50 aa) (STJ94956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PRKN antibody (390-440) {FITC} (STJ502121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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Anti-SIRT7 antibody (350-400) (STJ503042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Parkin Blocking Peptide peptide (STJ504532)

Applications :

Immunodepletion/Immunocompetition

£260.50

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SIRT7 Antibody Positve Control Positive Control peptide (STJ505156)

Applications :

WB

£308.50

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Human AURKB (Aurora Kinase B) Sandwich ELISA Kit (STJE0007403)

Applications :

ELISA

Reactivity :

Human

£421.50 - £586.50

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Human PARK2 (Parkinson Disease Protein 2) Sandwich ELISA Kit (STJE0011281)

Applications :

ELISA

Reactivity :

Human

£457.50 - £636.50

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Human AURKB (Aurora Kinase B) Sandwich ELISA Kit Ready-To-Use Kit (STJE0013779)

Applications :

ELISA

Reactivity :

Human

£440.50 - £614.50

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Anti-PRKN antibody (N-Term) (STJA0005802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£488.50

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