St John's Laboratory Ltd

Western blot analysis of lysates from Mouse brain, using Phospho-Tau-T231 Rabbit pAb (STJ11101057) at 1:1000 dilution. Mouse brain were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPT-Thr231 antibody (STJ11101057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£126.50 - £618.50

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Immunofluorescence analysis of paraffin-embedded Rat brain using Phospho-Tau-Ser404 Rabbit pAb (STJ11101134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-MAPT-Ser404 antibody (STJ11101134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from SH-SY5Y and C6 cells, using Phospho-Tau-S404 Rabbit pAb (STJ22272) at 1:2000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100nM) for 1 hour and Calyculin A (100nM) for 30 minutes after serum-starvation overnight. C6 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-MAPT-S404 antibody (STJ22272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates, using PKR/EIF2AK2 Rabbit polyclonal antibody (STJ27547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.

Anti-EIF2AK2 antibody (1-270) (STJ27547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-CEBPB antibody [EBP-333] (STJ16101065)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£307.50 - £1,449.50

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Western blot analysis of various lysates, using Phospho-Tau-T181 Rabbit pAb (STJ11103760) at 1:600 dilution. Mouse brain cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Tau-T181 antibody (STJ11103760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-PKR/EIF2AK2-T446 Rabbit pAb (STJ11103768) at 1:2000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PKR/EIF2AK2-T446 antibody (STJ11103768)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Anti-DAB2IP antibody (STJA0007518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of mouse brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MAPT-Ser717/400 antibody (STJA0010037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of recombinant Human p-Tau217 Protein using Phospho-Tau-T217 Rabbit mAb (STJ11105851) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 100ng Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 5s.

Anti-Phospho-Tau-T217 antibody [S5851RM] (STJ11105851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£143.50 - £618.50

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Anti-Recombinant-PRKCE antibody [RM2C12] (STJA0025184)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Microtubule-associated protein tau antibody (Full-Length) (STJA0040700)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ICC/WB

Reactivity :

Bovine/Chicken/Horse/Human/Mouse/Pig/Rat

£528.50

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Phospho-Tau Ser198 antibody western blot human fetal brain STJA0033936

Anti-Phospho-Tau-Ser198 antibody [R03-7A2] (STJA0033936)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Phospho-Tau Ser202/Thr205 antibody western blot human fetal brain STJA0033946

Anti-Phospho-Tau-Ser202/Thr205 antibody [R09-3G2] (STJA0033946)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-DAB2IP antibody (STJA0004493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

£139.50 - £403.50

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Immunofluorescence analysis of PC-12 cells using DAB2IP Rabbit polyclonal antibody (STJ11103982) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DAB2IP antibody (205-320) (STJ11103982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using RhoA antibody (STJ25351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RHOA antibody (94-193) (STJ25351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using RhoA antibody (STJ118088) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RHOA antibody (1-193) (STJ118088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunohistochemistry analysis of RhoA in paraffin-embedded human placenta using RhoA Rabbit polyclonal antibody (STJ115882) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-RHOA antibody (94-193) (STJ115882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western Blot analysis of mouse kidney, rat kidney. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJ99666 was diluted at 1:10000

Anti-Phospho-RHOA-Ser188 antibody (STJA0009821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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RHOA antibody western blot HeLa lysates STJA0035443

Anti-RHOA antibody [R66-2G-8] (STJA0035443)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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SDS-PAGE of recombinant human RhoA protein 3µg under reducing conditions with Coomassie blue stain

Human RhoA protein (Recombinant) (His-Tag) (STJP016987)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Western blot analysis of extracts of HUVEC cells, using RhoA antibody (STJ11101643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RHOA antibody (1-100) [S3MR] (STJ11101643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tip60 (Phospho-Ser90) Antibody

Anti-Phospho-KAT5-Ser90 antibody (56-105 aa) (STJ91271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using TIP60 monoclonal antibody against truncated TIP60 recombinant protein.

Anti-KAT5 antibody [3F9] (STJ98422)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Human Phospho-MAPT (Microtubule-associated protein tau) Sandwich ELISA Kit (STJE0003815)

Applications :

ELISA

Reactivity :

Human

£161.50 - £450.50

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Human KAT5 protein (Recombinant) (N-His) (STJP005810)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Immunofluorescence analysis of HeLa cells, using TIP60 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-KAT5 antibody (52-101 aa) (STJ96029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of Jurkat cells using Phospho-TIP60 (S86) Polyclonal Antibody STJ90502

Anti-Phospho-KAT5-Ser86 antibody (52-101 aa) (STJ90502)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TIP60 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-KAT5 antibody (371-420 aa) (STJ96030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, antibody was diluted at 1:1000. Secondary antibody was STJ99631

Anti-KAT5 antibody (STJ99631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Human MAPT (Microtubule-associated protein tau) Sandwich ELISA Kit (STJE0002615)

Applications :

ELISA

Reactivity :

Human

£161.50 - £450.50

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Western Blot analysis of 3T3 cells using TIP60 Polyclonal Antibody. Secondary antibody was diluted STJ96031

Anti-KAT5 antibody (56-105 aa) (STJ96031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-HSF1 antibody western blot HeLa heat-treated lysates STJA0035659

Anti-Phospho-HSF1-Ser326 antibody [R77-7H-8] (STJA0035659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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HSF1 antibody western blot HeLa lysates STJA0014242

Anti-HSF1 antibody (485-529 aa) [R09-3M8] (STJA0014242)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-HSF1 antibody [SRM14695] (STJ11109425)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-HSF1-S326 Rabbit pAb (STJ11103729) at 1:500 dilution. MCF7 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-HSF1-S326 antibody (STJ11103729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various lysates, using Phospho-HSF1-S307 antibody (STJ11105865) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-HSF1-S307 antibody (STJ11105865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-HSF1-Ser303/Ser307 antibody [R01-7G4] (STJA0035072)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£140.50 - £308.50

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Immunofluorescence analysis of U2OS cells using HSF1 Rabbit polyclonal antibody (STJ115712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSF1 antibody (350-529) (STJ115712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HSF1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-HSF1 antibody (497-510 aa) [R07-0G0] (STJA0011457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£140.50 - £308.50

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SDS-PAGE gel of recombinant human HSF1 protein 3μg under reducing conditions Coomassie stain

Human HSF1 protein (Recombinant) (His-Tag) (STJP019655)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Anti-Phospho-HSF1-Ser326 antibody [R08-4I1] (STJA0034439)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£140.50 - £308.50

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Western blot analysis of lysates from 293T cells, using Phospho-HSF1-S326 Rabbit mAb (STJ11102636) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-HSF1-S326 antibody [S6MR] (STJ11102636)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human colon using HSF1 Rabbit polyclonal antibody (STJ11104640) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-HSF1 antibody (367-529) (STJ11104640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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STJ70714 (0. 3µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT2 antibody (C-Term) (STJ70714)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Mouse PKBb (Protein Kinase B Beta) Sandwich ELISA Kit (STJE0011492)

Applications :

ELISA

Reactivity :

Mouse

£413.50 - £575.50

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Mouse PKBb (Protein Kinase B Beta) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017878)

Applications :

ELISA

Reactivity :

Mouse

£432.50 - £602.50

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