• Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-KAT5 antibody (STJ99631)

SKU:
STJ99631

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone acetyltransferase KAT5-interacting protein is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KAT5
Gene ID: 10524
Uniprot ID: KAT5_HUMAN
Specificity: This antibody detects endogenous levels of Tip60.
Immunogen: Synthesized peptide derived from the human Tip60 Polyclonal
Function Catalytic subunit of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H2A and H4. Histone acetylation alters nucleosome-DNA interactions and promotes interaction of the modified histones with other proteins which positively regulate transcription. The NuA4 histone acetyltransferase complex is required for the activation of transcriptional programs associated with proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR): the complex inhibits TP53BP1 binding to chromatin via MBTD1, which recognizes and binds histone H4 trimethylated at 'Lys-20' (H4K20me), and KAT5 that catalyzes acetylation of 'Lys-15' of histone H2A (H2AK15ac), thereby blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks. Also involved in DSB repair by mediating acetylation of 'Lys-5' of histone H2AX (H2AXK5ac), promoting NBN/NBS1 assembly at the sites of DNA damage. The NuA4 complex plays a key role in hematopoietic stem cell maintenance and is required to maintain acetylated H2A.Z/H2AZ1 at MYC target genes. The NuA4 complex is also required for spermatid development by promoting acetylation of histones: histone hyperacetylation is required for histone replacement during the transition from round to elongating spermatids. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Also acetylates non-histone proteins, such as BMAL1, ATM, AURKB, CHKA, CGAS, ERCC4/XPF, LPIN1, NDC80/HEC1, NR1D2, RAN, SOX4, FOXP3, ULK1 and RUBCNL/Pacer. Directly acetylates and activates ATM. Promotes nucleotide excision repair (NER) by mediating acetylation of ERCC4/XPF, thereby promoting formation of the ERCC4-ERCC1 complex. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Acts as a regulator of regulatory T-cells (Treg) by catalyzing FOXP3 acetylation, thereby promoting FOXP3 transcriptional repressor activity. Involved in skeletal myoblast differentiation by mediating acetylation of SOX4. Catalyzes acetylation of APBB1/FE65, increasing its transcription activator activity. Promotes transcription elongation during the activation phase of the circadian cycle by catalyzing acetylation of BMAL1, promoting elongation of circadian transcripts. Together with GSK3 (GSK3A or GSK3B), acts as a regulator of autophagy: phosphorylated at Ser-86 by GSK3 under starvation conditions, leading to activate acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer. Acts as a regulator of the cGAS-STING innate antiviral response by catalyzing acetylation the N-terminus of CGAS, thereby promoting CGAS DNA-binding and activation. Also regulates lipid metabolism by mediating acetylation of CHKA or LPIN1. Promotes lipolysis of lipid droplets following glucose deprivation by mediating acetylation of isoform 1 of CHKA, thereby promoting monomerization of CHKA and its conversion into a tyrosine-protein kinase. Acts as a regulator of fatty-acid-induced triacylglycerol synthesis by catalyzing acetylation of LPIN1, thereby promoting the synthesis of diacylglycerol. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA) and 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA), and is able to mediate protein crotonylation and 2-hydroxyisobutyrylation, respectively. Acts as a key regulator of chromosome segregation and kinetochore-microtubule attachment during mitosis by mediating acetylation or crotonylation of target proteins. Catalyzes acetylation of AURKB at kinetochores, increasing AURKB activity and promoting accurate chromosome segregation in mitosis. Acetylates RAN during mitosis, promoting microtubule assembly at mitotic chromosomes. Acetylates NDC80/HEC1 during mitosis, promoting robust kinetochore-microtubule attachment. Catalyzes crotonylation of MAPRE1/EB1, thereby ensuring accurate spindle positioning in mitosis.
Protein Name Histone Acetyltransferase Kat5
60 Kda Tat-Interactive Protein
Tip60
Histone Acetyltransferase Htatip
Hiv-1 Tat Interactive Protein
Lysine Acetyltransferase 5
Protein 2-Hydroxyisobutyryltransferase Kat5
Protein Acetyltransferase Kat5
Protein Crotonyltransferase Kat5
Cpla(2-Interacting Protein
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-2559586
Reactome: R-HSA-3214847
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693548
Reactome: R-HSA-5693554
Reactome: R-HSA-5693565
Reactome: R-HSA-5693568
Reactome: R-HSA-5693571
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9018519
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Reactome: R-HSA-9733709
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Centromere
Kinetochore
Cytoskeleton
Spindle Pole
Nucleolus
Perinuclear Region
Upon Stimulation With Edn1
It Is Exported From The Nucleus To The Perinuclear Region And Uv Irradiation Induces Translocation Into Punctuate Subnuclear Structures Named Nuclear Bodies
Transiently Localizes To Kinetochores In Early Mitosis
Localizes To Spindle Poles When Chromosomes Align During Metaphase
Localizes In The Cytoplasm And Nucleus Of Round Spermatids
Alternative Antibody Names Anti-Histone Acetyltransferase Kat5 antibody
Anti-60 Kda Tat-Interactive Protein antibody
Anti-Tip60 antibody
Anti-Histone Acetyltransferase Htatip antibody
Anti-Hiv-1 Tat Interactive Protein antibody
Anti-Lysine Acetyltransferase 5 antibody
Anti-Protein 2-Hydroxyisobutyryltransferase Kat5 antibody
Anti-Protein Acetyltransferase Kat5 antibody
Anti-Protein Crotonyltransferase Kat5 antibody
Anti-Cpla(2-Interacting Protein antibody
Anti-KAT5 antibody
Anti-HTATIP antibody
Anti-TIP60 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance