Vesicle-Mediated Transport | Research Area | St John's Laboratory

Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-AKT1 Thr450 antibody western blot U2OS C6 STJA0035050

Anti-Phospho-AKT1-Thr450 antibody [R07-4A7] (STJA0035050)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Phospho-AKT1 antibody western blot 3T3 lysates STJA0035669

Anti-Phospho-AKT1-Thr450 antibody [R72-1U-9] (STJA0035669)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit polyclonal antibody (STJ11105868) at 1:900 dilution. 293F cells, 293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT2-S474 antibody (STJ11105868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£143.50 - £618.50

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Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1：500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Alpha 6-Tubulin-Ser-165 antibody (STJA0041427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Phospho-alpha Tubulin Tyr272 antibody western blot Jurkat STJA0035837

Anti-Phospho-alpha Tubulin-Tyr272 antibody [R06-9Z1] (STJA0035837)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Phospho-AMPK alpha 2 antibody western blot 293T STJA0036159

Anti-Phospho-AMPK alpha 2-Ser345 antibody [R04-5T2] (STJA0036159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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AMPK beta 1 antibody western blot 293T cells STJA0036166

Anti-Phospho-AMPK alpha 2-Ser491 antibody [R05-9Q9] (STJA0036166)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-AMPK alpha antibody western blot mouse heart STJA0035991

Anti-Phospho-AMPK alpha 2-Thr172 antibody [R03-6K2] (STJA0035991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Amyloid Precursor Protein antibody immunocytochemistry LNcap STJA0035052

Anti-Phospho-Amyloid Precursor Protein-Thr743 antibody [R07-4D0] (STJA0035052)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 Rabbit pAb (STJ117921) at 1:1000 dilution. 293T cells were treated by Calyculin A for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Hydrogen Peroxide (2nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-AP2M1-T156 antibody (STJ117921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-AP2M1-Thr156 antibody (STJ194700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Phospho-AP2M1 Thr156 antibody western blot K562 STJA0034025

Anti-Phospho-AP2M1-Thr156 antibody [R03-8C7] (STJA0034025)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from Mouse brain, using Phospho-APP-T668 Rabbit pAb (STJ22017) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-APP-T668 antibody (STJ22017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-APP-Thr668 antibody (STJ196240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Amyloid-Beta (T743) Polyclonal Antibody

Anti-Phospho-APP-Thr743 antibody (711-760 aa) (STJ91015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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WB analysis A431 cells Arp2 detection with phosphatase treatment comparison STJA0041071

Anti-Phospho-Arp2-T237/T238 antibody (STJA0041071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ARRB1-Ser412 antibody (369-418 aa) (STJ90802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Phospho-AS160 antibody western blot 293T cells STJA0035703

Anti-Phospho-AS160-Thr642 antibody [R83-5T-8] (STJA0035703)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Phospho-beta Arrestin 1 Ser412 antibody western blot A549 MCF-7 U251 HL-60 STJA0035053

Anti-Phospho-beta Arrestin 1-Ser412 antibody [R05-6F7] (STJA0035053)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Beta-Tubulin-Ser-172 antibody (STJA0041423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Western blot analysis human Jurkat cells using anti-c-Cbl Tyr-700 antibody STJA0041109

Anti-Phospho-c-Cbl-Tyr-700 antibody [M161] (STJA0041109)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Phospho-CBL Ser669 antibody western blot K562 STJA0034121

Anti-Phospho-CBL-Ser669 antibody [R04-2C9] (STJA0034121)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Phospho-CBL antibody western blot HeLa lysates STJA0035684

Anti-Phospho-CBL-Ser669 antibody [R48-7F-7] (STJA0035684)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-CBL-Tyr-700 antibody [M161] (STJA0005316)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CBL (Phospho-Tyr674) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CBL-Tyr674 antibody (640-689 aa) (STJ90807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CBL (Phospho-Tyr700) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CBL-Tyr700 antibody (666-715 aa) (STJ91036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 K562 treated with LPS, 2 K562, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CBL-Tyr731 antibody (STJ196267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of A549 cells, using CBL (Phospho-Tyr774) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CBL-Tyr774 antibody (740-789 aa) (STJ91035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-CBL Tyr774 antibody western blot K562 C6 HeLa STJA0033922

Anti-Phospho-CBL-Tyr774 antibody [R07-8A3] (STJA0033922)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y700 Rabbit pAb (STJ116400) at 1:2000 dilution or CBL antibody (STJ22913). Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CBL-Y700 antibody (STJ116400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y774 Rabbit pAb (STJ22913). Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30S.

Anti-Phospho-CBL-Y774 antibody (STJ117892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using CD4 (Phospho-Ser433) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CD4-Ser433 antibody (401-450 aa) (STJ91298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CFTR-Ser737 antibody (STJ193221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of LOVO+H2O2 cells using Phospho-Cortactin (Y421) Polyclonal Antibody STJ90232

Anti-Phospho-CTTN-Tyr421 antibody (388-437 aa) (STJ90232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Cortactin (phospho Tyr466) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-CTTN-Tyr466 antibody (441-490 aa) (STJ91006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Phospho-Dishevelled 2 Ser143 antibody western blot K562 STJA0034249

Anti-Phospho-Dishevelled 2-Ser143 antibody [R02-6I1] (STJA0034249)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of mouse cells using Phospho-Dynamin I (S774) Polyclonal Antibody

Anti-Phospho-DNM1-Ser774 antibody (740-789 aa) (STJ90787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of Mouse-Brain cells using Phospho-Dynamin I (S778) Polyclonal Antibody

Anti-Phospho-DNM1-Ser778 antibody (751-800 aa) (STJ90607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-S1070 Rabbit pAb (STJ22101) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-EGFR-S1070 antibody (STJ22101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-EGFR-Ser-1142 antibody (STJA0005396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot A431 cells EGFR phosphorylation detection using antibody STJA0041202

Anti-Phospho-EGFR-Ser-1142 antibody (STJA0041202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-EGFR-Ser-967 antibody (STJA0005397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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