Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using E2F3 antibody (STJ111428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-E2F3 antibody (200-300) (STJ111428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using E2F4 antibody (STJ11103078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-E2F4 antibody (250-350) [S8MR] (STJ11103078)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of Jurkat cells using E2F-4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F4 antibody (61-110 aa) (STJ96687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-E2F4 antibody (89-198) (STJ11103181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of lysates from A-549 cells, using E2F5 Rabbit polyclonal antibody (STJ118482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-E2F5 antibody (197-346) (STJ118482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of HeLa cells using E2F-5 Polyclonal Antibody

Anti-E2F5 antibody (93-142 aa) (STJ92810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using EBAG9 antibody (STJ23462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-EBAG9 antibody (28-213) (STJ23462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-EBAG9 antibody (STJA0008643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using ECE1 Rabbit polyclonal antibody (STJ11104569) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ECE1 antibody (670-770) (STJ11104569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from normal (control) and ECE1 knockout (KO) HeLa cells, using ECE1 antibody (STJ27605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ECE1 antibody (670-770) (STJ27605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-ECE1 antibody (STJ192263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using EDG2/LPA-1 Rabbit monoclonal antibody (STJ11103079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EDG2/LPA-1 antibody (1-100) [S9MR] (STJ11103079)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of mouse lung cells using ET-1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-EDN1 antibody (131-180 aa) (STJ96512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and EDN1 knockout (KO) HeLa cells, using [KO Validated] EDN1 Rabbit polyclonal antibody (STJ119977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-EDN1 antibody (18-212) (STJ119977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Endothelin 1 Rabbit polyclonal antibody (STJ23471) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EDN1 antibody (18-212) (STJ23471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-EDN2 antibody (69-178 aa) (STJ11107483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EDN2 antibody (STJ192244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of A-549 cells, using EDN3 antibody (STJ26165) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 20s.

Anti-EDN3 antibody (17-187) (STJ26165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-EDN3 antibody (17-238) (STJ115909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of various cells using ETAR Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-EDNRA antibody (378-427 aa) (STJ93004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Anti-EDNRA antibody (C-Term) (STJ13101085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of extracts of various cell lines, using EDNRB antibody (STJ23473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-EDNRB antibody (1-100) (STJ23473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human Prostate Tissue using Endothelin B ReceptorRabbit pAb diluted at 1:200.

Anti-EDNRB antibody (270-350 aa) (STJ197396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of Hela cells using ETBR Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-EDNRB antibody (31-80 aa) (STJ98617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-EDNRB antibody (C-Term) (STJ13100124)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Immunofluorescence analysis of U-2 OS cells using EED Rabbit polyclonal antibody (STJ114646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EED antibody (1-100) (STJ114646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using EED rabbit polyclonal antibody (STJ27324) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EED antibody (1-400) (STJ27324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-EED antibody (STJA0006915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using eEF2K Polyclonal Antibody diluted at 1：500

Anti-EEF2K antibody (331-380 aa) (STJ92839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A-549 cells using EEF2K antibody (STJ27357). Blue: DAPI for nuclear staining.

Anti-EEF2K antibody (406-725) (STJ27357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-EEF2K antibody (C-Term) (STJA0005388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£319.50

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EEPD1 antibody (140-190 aa) (STJ193624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-EFCAB7 antibody (170-400 aa) (STJ11106131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EFCAB7 antibody (386-436 aa) (STJ193828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from mouse lung, using EFHD2 Rabbit polyclonal antibody (STJ117125) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EFHD2 antibody (1-78) (STJ117125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EFHD2 antibody (13-63 aa) (STJ195399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-EGF antibody (1000-1060 aa) (STJ98714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of human skin using EGF Rabbit polyclonal antibody (STJ115574) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EGF antibody (901-916) (STJ115574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Dot-blot analysis of purified mature EGF using EGF antibody (STJ23488) at 1:500 dilution NA

Anti-EGF antibody (971-1023) (STJ23488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis using EGF monoclonal antibody against EGF-hIgGFc transfected HEK293 cell lysate.

Anti-EGF antibody [4E11] (STJ98013)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Standard Curve for Human EGF: Capture Antibody Mouse mAb 201165-5E9 (5E9-A10-A6) to Human EGF at 4 Mu g/ml and Detector Antibody Mouse mAb 201165-7C11 (7C11-G10-H8) to Human EGF at 1 Mu g/ml.

Anti-EGF antibody [7C11-G10-H8] (STJ99272)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-EGF Receptor-E746-A750del Specific antibody (STJ11106934)

Clonality :

Polyclonal

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of A431, using EGFR rabbit polyclonal antibody (STJ113093) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EGFR antibody (1021-1210) (STJ113093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g EGFR antibody (STJ113769). Western blot was performed from the immunoprecipitate using EGFR antibody (STJ113769) at a dilution of 1:1000.

Anti-EGFR antibody (1021-1210) (STJ113769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A-431 cells using [KD Validated] EGFR Rabbit polyclonal antibody (STJ113840) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EGFR antibody (1021-1210) (STJ113840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (STJ113701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EGFR antibody (1026-1126) (STJ113701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunocytochemistry analysis of EGFR (green) in 3T3 using EGFR antibody, and DAPI (blue).

Anti-EGFR antibody (1075-1093 aa) [R01-2D3] (STJA0012677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£264.50

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Immunocytochemistry analysis of EGFR (green) in Hela using EGFR antibody, and DAPI (blue)

Anti-EGFR antibody (1075-1093 aa) [R03-6B1] (STJA0013018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£264.50

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