Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of lysates from wild type (WT) and PAK2 knockout (KO) HeLa cells, using [KO Validated] PAK2 Rabbit polyclonal antibody (STJ11100066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PAK2 antibody (1-380) (STJ11100066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using PAK2 antibody (STJ29472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PAK2 antibody (120-240) (STJ29472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 cells using PAK Gamma Polyclonal Antibody

Anti-PAK2 antibody (5-54 aa) (STJ94946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using PAK Gamma monoclonal antibody against HeLa (1) , Jurkat (2) , A549 (3) , HEK293 (4) and K562 (5) cell lysate.

Anti-PAK2 antibody [3B5] (STJ98304)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£149.00 - £360.00

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PAK2 antibody western blot K562 rat brain C6 3T3 HeLa STJA0034687

Anti-PAK2 antibody [R03-7C6] (STJA0034687)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-PAK2 antibody [SRM13614] (STJ11108344)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of NIH-3T3 cells using PAK3 Rabbit polyclonal antibody (STJ11101194) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PAK3 antibody (1-100) (STJ11101194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using PAK3 Rabbit monoclonal antibody (STJ11101854) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PAK3 antibody (1-100) [S4MR] (STJ11101854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-PAK3 antibody (121-170 aa) (STJ94945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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PAK3 antibody western blot human fetal brain STJA0036598

Anti-PAK3 antibody [R05-7G2] (STJA0036598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of LNCaP cells, using PAK4 antibody (STJ113249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PAK4 antibody (450-550) (STJ113249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of lysates from U-87MG cells, using PAK5 Rabbit polyclonal antibody (STJ118317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PAK5 antibody (600-700) (STJ118317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various cells using PAK5 Polyclonal Antibody

Anti-PAK5 antibody (661-710 aa) (STJ94938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using PAK6 Rabbit polyclonal antibody (STJ110131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PAK6 antibody (1-270) (STJ110131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PAK6 antibody (100-180 aa) (STJ190138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-PAK6 antibody (N-Term) (STJA0005510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£367.50

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Western blot PAK6 phosphorylation with anti-PAK6 N-terminal and Ser-165 antibodies STJA0041337

Anti-PAK6 antibody (N-Term) (STJA0041337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£542.50

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Anti-Pan-Alpha Tubulin antibody [SM5020] (STJA0005020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Immunohistochemistry analysis of paraffin embedded human pancreas using Pan-cadherin at dilution of 1:200

Anti-Pan-Cadherin antibody [SM4933] (STJA0004933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Anti-Pan-MAGUK antibody [K28/86] (STJA0019763)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat/Drosophila

£198.50 - £1,170.50

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Anti-Pan-PKC antibody [SM5008] (STJA0005008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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ICC IF of RAS in HeLa cells stained with STJA0041606 antibody Alexa Fluor 488 secondary green DAPI nucleus blue

Anti-pan-RAS antibody (1-186aa) [AT2G9] (STJA0041606)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Western blot showing human pan-RAS antibody detection of NRAS KRAS and HRAS recombinant proteins

Anti-pan-RAS antibody (1-186aa) [AT2G9] (STJA0041946)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/FACS

Reactivity :

Human

£238.50 - £361.50

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Anti-Pan-RhO antibody (50-100 aa) (STJ13100783)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Anti-Pan-RhO antibody (50-100 aa) (STJ13100811)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Anti-Pan-RhO antibody (50-100 aa) (STJ13100830)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Anti-Pan-RhO antibody (STJ13101904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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PAR2 antibody western blot K562 lysates STJA0037163

Anti-PAR2 antibody [R06-4Y1] (STJA0037163)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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PAR6 antibody western blot Jurkat STJA0036697

Anti-PAR6 antibody [R03-5L4] (STJA0036697)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Parathyroid hormone (PTH) antibody IHC Human parathyroid gland STJ180312

Anti-Parathyroid hormone antibody (1-34aa) [ZM207] (STJ180312)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£149.50 - £582.50

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Anti-Parathyroid Hormone antibody (STJA0037797)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£244.50 - £335.50

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Parathyroid Hormone antibody immunohistochemistry parathyroidoma STJA0033486

Anti-Parathyroid Hormone antibody [10A7-9G5-10A10] (STJA0033486)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£214.50 - £308.50

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Anti-Parathyroid Hormone antibody [BGN/1F8] (STJ16101942)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/IHC-P

Reactivity :

Human/Rat/Mouse

£819.50

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Anti-Parathyroid Hormone antibody [IHC206] (STJA0042237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

£148.50 - £398.50

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Anti-Parathyroid Hormone antibody [IHC645] (STJA0042057)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

£148.50 - £398.50

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Anti-Parathyroid Hormone antibody [MRQ-31] (STJ16100412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£845.50 - £1,423.50

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Parathyroid Hormone antibody western blot human recombinant protein STJA0035466

Anti-Parathyroid Hormone antibody [R83-6R-7] (STJA0035466)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

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Parathyroid Hormone Receptor 1 antibody western blot HeLa STJA0036649

Anti-Parathyroid Hormone Receptor 1 antibody [R05-7Y8] (STJA0036649)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various cells using PARD3A Polyclonal Antibody

Anti-PARD3 antibody (1141-1190 aa) (STJ94955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using PARD6A Rabbit polyclonal antibody (STJ24893) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PARD6A antibody (1-100) (STJ24893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of lysates from Jurkat cells, using PARD6B Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PARD6B antibody (233-282 aa) (STJ94954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-PARD6B antibody (300-350) (STJ506125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-PARD6B antibody (300-350) {Biotin} (STJ506126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-PARD6B antibody (300-350) {FITC} (STJ506127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-PARD6B antibody (340-372) (STJ506140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-PARD6B antibody (340-372) {Biotin} (STJ506158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-PARD6B antibody (340-372) {FITC} (STJ506159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of extracts of various cell lines, using Cleaved PARP1 p25 antibody (STJ11101731) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PARP1 antibody (150-214) [S1MR] (STJ11101731)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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