Protein Localisation | Research Area | St John's Laboratory

Western blot analysis of lysates from C6 cells using Ubiquitin Rabbit polyclonal antibody (STJ26009) at 1:900 dilution. C6 cells were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-229) (STJ26009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of HepG2 cells using UBC Rabbit polyclonal antibody (STJ29897) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBC antibody (1-100) (STJ29897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBC antibody (160-210 aa) (STJ194141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-UBC/polyUb/Polyubiquitin-C antibody [SAA0328] (STJA0019255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using UBE2D1 antibody (STJ26014) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-UBE2D1 antibody (1-147) (STJ26014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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SFT antibody western blot Raw2647 STJA0012589

Anti-UBE2D1 antibody (119-132 aa) [R07-4E8] (STJA0012589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Immunohistochemistry analysis of SFT in paraffin-embedded rat ovary using SFT Rabbit monoclonal antibody (STJ11104166) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-UBE2D1 antibody (50-147) [S4166RM] (STJ11104166)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBE2D1 antibody (STJ191590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using UBE2D2 Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000

Anti-UBE2D2 antibody (98-147 aa) (STJ96163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using UBE2D3 Rabbit polyclonal antibody (STJ116847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-UBE2D3 antibody (1-100) (STJ116847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBE2D3 antibody (STJ191591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Ubiquitin antibody (STJA0040904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Ubiquitin antibody western blot HeLa 3T3 rat brain STJA0032907

Anti-Ubiquitin antibody [1B8-8E7-1H7] (STJA0032907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

$244.95 - $360.87

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Ubiquitin antibody western blot HeLa 3T3 rat brain STJA0032914

Anti-Ubiquitin antibody [7F2-4B1-10D3] (STJA0032914)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

$184.23 - $388.47

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Ubiquitin antibody immunocytochemistry CEM cells STJA0034973

Anti-Ubiquitin antibody [R01-7F1] (STJA0034973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Rat

$184.23 - $388.47

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Ubiquitin antibody western blot 293 cells STJA0034974

Anti-Ubiquitin antibody [R02-4I6] (STJA0034974)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

$184.23 - $388.47

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Anti-Ubiquitin antibody [SRM13312] (STJ11108042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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UBE2D3 antibody western blot K562 rat brain C6 3T3 HeLa STJA0034972

Anti-Ubiquitin Conjugating Enzyme E2 D3 antibody [R01-4J4] (STJA0034972)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Ubiquitin K48 antibody western blot C6 C2C12 STJA0034497

Anti-Ubiquitin K48 antibody [R06-2H7] (STJA0034497)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Ubiquitin K63 antibody western blot K63linkedUb2 recombinant protein STJA0036235

Anti-Ubiquitin K63 antibody [R06-2A6] (STJA0036235)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Ubiquitin K63 antibody western blot HeLa A549 STJA0035398

Anti-Ubiquitin K63 antibody [R06-2H7] (STJA0035398)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g UBL4A antibody (STJ26569). Western blot was performed from the immunoprecipitate using UBL4A antibody (STJ26569) at a dilution of 1:1000.

Anti-UBL4A antibody (1-157) (STJ26569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-UBL4A antibody (N-Term) (STJA0010316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using USP9X Rabbit polyclonal antibody (STJ118737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-USP9X antibody (1-100) (STJ118737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using USP9X Rabbit mAb (STJ11102548) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-USP9X antibody (1-100) [S8MR] (STJ11102548)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-USP9X antibody (STJA0006795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-USP9X antibody [2G7] (STJA0032733)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Monkey/Mouse/Rat

$309.81 - $425.73

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USP9X antibody western blot HeLa lysates STJA0036843

Anti-USP9X antibody [R09-5B7] (STJA0036843)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-VAMP 2, 1 antibody (10-60 aa) (STJ13100328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$445.05

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Anti-VAMP 2, 1 antibody (10-60) (STJ13100324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

$445.05

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Anti-VAMP-2 antibody (1-89aa) [3E5] (STJA0041961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$329.13 - $498.87

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Anti-VAMP-2 antibody (1-89aa) [3E5] (STJA0041986)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human/Mouse

$302.91 - $458.85

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using VAMP2 antibody (STJ26068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-VAMP2 antibody (1-100) (STJ26068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Confocal imaging of paraffin-embedded Mouse brain using VAMP2 Rabbit monoclonal antibody (STJ11102024, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.

Anti-VAMP2 antibody (1-100) [S4MR] (STJ11102024)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-VAMP2 antibody (STJ13102600)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$445.05

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Anti-VAMP2 antibody (STJ13102601)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$445.05

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Anti-VAMP2 antibody (STJA0006851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-VAMP2 antibody [PT1251R] (STJA0040129)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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VAMP2 antibody immunocytochemistry Jurkat cells STJA0034985

Anti-VAMP2 antibody [R04-7A5] (STJA0034985)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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VAMP2 antibody western blot PC-12 mouse brain STJA0035401

Anti-VAMP2 antibody [R05-7F2] (STJA0035401)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-VAMP2 antibody [SAA1655] (STJA0020083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$273.93 - $1,615.29

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Immunohistochemistry analysis of VAPA in paraffin-embedded human stomach using VAPA Rabbit polyclonal antibody (STJ114805) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-VAPA antibody (137-227) (STJ114805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-VAPA antibody (34-84 aa) (STJ195181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Confocal imaging of HeLa cells using VDAC1 Rabbit monoclonal antibody (STJ11101777, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-VDAC1 antibody (1-100) [S7MR] (STJ11101777)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of extracts from wild type (WT) and VDAC1 knockout (KO) 293T (KO) cells, using VDAC1 antibody (STJ11104504) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-VDAC1 antibody (1-283) (STJ11104504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunohistochemistry analysis of VDAC1/ Porin in paraffin-embedded human stomach using VDAC1/ Porin Rabbit polyclonal antibody (STJ118181) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-VDAC1 antibody (1-283) (STJ118181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using VDAC1/ Porin antibody (STJ26080) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VDAC1 antibody (1-283) (STJ26080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysate from K562 cells, using VDAC1 Antibody.

Anti-VDAC1 antibody (1-50 aa) (STJ96755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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