Programmed Cell Death | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Cleaved-CASP9-D315 antibody (266-315 aa) (STJ90013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp330) Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Cleaved-CASP9-D330 antibody (281-330 aa) (STJ90012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Cleaved-DFFA-Ser118 antibody (117-118) (STJA0005851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Cleaved-DFFA-Thr225 antibody (224-225) (STJA0005852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from THP-1 cells using Cleaved GSDMD (N Terminal) monoclonal antibody (STJ11105290) at 1:1000 dilution. THP-1 cells were treated by LPS+Nigericin. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-Cleaved-GSDMD antibody (N-Term) [S5290RM] (STJ11105290)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Cleaved-IL-18-Tyr37 antibody [SRM] (STJ11107139)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of recombinant Human IL-18 Protein using Cleaved IL-18 (Tyr37) Rabbit monoclonal antibody (STJ11105289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 1 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Cleaved-IL18-Tyr37 antibody [S5289RM] (STJ11105289)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various cells using Cleaved-Spectrin Alpha II (D1185) Polyclonal Antibody diluted at 1：500

Anti-Cleaved-SPTAN1-D1185 antibody (1136-1185 aa) (STJ90073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CLSPN antibody (10-90 aa) (STJ190129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CLSPN Rabbit polyclonal antibody (STJ119393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 150s.

Anti-CLSPN antibody (650-750) (STJ119393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g Beta-Catenin antibody (STJ11103800). Western blot was performed from the immunoprecipitate using Beta-Catenin antibody (STJ11103800) at a dilution of 1:1000.

Anti-CTNNB1 antibody (1-100) (STJ11103800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £642.50

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Immunofluorescence analysis of U2OS cells using Beta-Catenin antibody (STJ113475) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CTNNB1 antibody (1-100) (STJ113475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Beta-Catenin antibody (STJ11101750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CTNNB1 antibody (1-100) [S0MR] (STJ11101750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using Catenin-Beta Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (3-52 aa) (STJ92041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-CTNNB1 primary antibody (STJ500624) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CTNNB1 antibody (50-100) (STJ500624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CTNNB1 antibody (50-100) {Biotin} (STJ500626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CTNNB1 antibody (50-100) {FITC} (STJ500627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various cells using Catenin-Beta 1 Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (626-675 aa) (STJ92042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from normal (control) and Beta-Catenin knockout (KO) 293T cells, using Beta-Catenin antibody (STJ113088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CTNNB1 antibody (650-750) (STJ113088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Beta-Catenin antibody (STJ113822). Western blot was performed from the immunoprecipitate using Beta-Catenin antibody (STJ113822) at a dilution of 1:1000.

Anti-CTNNB1 antibody (667-781) (STJ113822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Beta-catenin antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 94 kDa

Anti-CTNNB1 antibody (700-781 aa) [ABT-Catenin] (STJ197008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunohistochemistry of mouse lung using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 antibody at 1:500:DAB detection.

Anti-CTNNB1 antibody (731aa C-Term) (STJ140021)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot with Anti-CTNNB1 Antibody (STJ500625) with peptide on at 1:500 dilution in buffer.

Anti-CTNNB1 antibody (740-790) (STJ500625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CTNNB1 antibody (740-790) {Biotin} (STJ500628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CTNNB1 antibody (740-790) {FITC} (STJ500629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CTNNB1 antibody (C-Term) [M118] (STJA0005301)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£390.50

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Anti-CTNNB1 antibody (N-Term) (STJA0005302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of 1) 3T3, 2) Rat Liver, diluted at 1:2,000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-CTNNB1 antibody (STJ197317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Rabbit

£48.00 - £252.00

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Anti-CTNNB1 antibody (STJA0009925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Catenin-Beta monoclonal antibody against CTNNB1-hIgGFc transfected HEK293 cell lysate.

Anti-CTNNB1 antibody [4D5] (STJ97896)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Catenin-Beta monoclonal antibody (4F2) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CTNNB1 antibody [4F2] (STJ97420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Zebrafish

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using Cytochrome C Rabbit polyclonal antibody (STJ115391) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CYCS antibody (1-105) (STJ115391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using Cytochrome C Rabbit polyclonal antibody (STJ23308) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CYCS antibody (1-105) (STJ23308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Cytochrome C in paraffin-embedded human appendix using Cytochrome C Rabbit monoclonal antibody (STJ11102194) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CYCS antibody (1-105) [S4MR] (STJ11102194)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of mouse-spleen tissue. 1, CYCS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-CYCS antibody (1-69 aa) (STJ98953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CYCS antibody [4B10] (STJ97419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Anti-DAP Kinase 1 antibody (1230-1430 aa) (STJ11106246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using DAP Kinase 1 (DAP Kinase 1 (DAPK1) ) Antibody (STJ28308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-DAPK1 antibody (1141-1430) (STJ28308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-DAPK1 antibody (1370-1420) (STJ500726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-DAPK1 antibody (1370-1420) {Biotin} (STJ500727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-DAPK1 antibody (1370-1420) {FITC} (STJ500728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using DAP Kinase 1 (DAPK1) Rabbit monoclonal antibody (STJ11101334) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-DAPK1 antibody (250-350) [S4MR] (STJ11101334)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis ofmouse braincells using DAPK1 Polyclonal Antibody diluted at 1：1000

Anti-DAPK1 antibody (274-323 aa) (STJ92652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of Rat skeletal muscle, using DAPK2 antibody (STJ110498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DAPK2 antibody (1-100) (STJ110498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-DAPK2 antibody (284-333 aa) (STJ92653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using DAPK3 Rabbit polyclonal antibody (STJ117241) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DAPK3 antibody (1-100) (STJ117241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using DAPK3 Polyclonal Antibody

Anti-DAPK3 antibody (241-290 aa) (STJ92654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using DBNL Rabbit polyclonal antibody (STJ115699) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DBNL antibody (131-430) (STJ115699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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