Programmed Cell Death | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Thr445) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADD1-Thr445 antibody (411-460 aa) (STJ91115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Anti-Phospho-Akt-Ser473 antibody [M114] (STJA0041048)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP

Reactivity :

Human/Mouse/Rat

$748.65

Choose Options

Phospho-AKT Ser473 antibody western blot C6 3T3 HeLa STJA0033931

Anti-Phospho-AKT-Ser473 antibody [R01-4H6] (STJA0033931)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Phospho-AKT Ser473 antibody western blot NIH/3T3 STJA0036228

Anti-Phospho-AKT-Ser473 antibody [R06-7C5] (STJA0036228)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Western blot WB analysis A431 cells Akt phosphorylation at Thr-34 STJA0041062

Anti-Phospho-Akt-Thr-34 antibody (STJA0041062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Rabbit/Rat

$748.65

Choose Options

Western blot with Anti-Phospho-AKT1 Antibody (STJ502089) with PAKT peptide.1:250 dilution in buffer. From 10% SDS gel.

Anti-Phospho-AKT1 antibody (430-480) (STJ502089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

$686.55

Choose Options

Anti-Phospho-AKT1 antibody (430-480) {Biotin} (STJ502090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

$761.07

Choose Options

Anti-Phospho-AKT1 antibody (430-480) {FITC} (STJ502091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

$761.07

Choose Options

Anti-Phospho-AKT1 antibody [M114] (STJA0005252)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$504.39

Choose Options

Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb (STJ11102574) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S124 antibody [S4MR] (STJ11102574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

Choose Options

Western blot analysis of lysates from 293T cells, using Phospho-AKT1-S129 Rabbit pAb (STJ11103780) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S129 antibody (STJ11103780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

Choose Options

Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb (STJ11102573) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S129 antibody [S3MR] (STJ11102573)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

Choose Options

Anti-Phospho-AKT1-S212 antibody [SAA0279] (STJA0019075)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

$269.79 - $1,820.91

Choose Options

Western Blot (WB): Xiao, Jun, et al. "Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt-SREBP-2 signaling STJ97727

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$67.62 - $360.18

Choose Options

Western blot analysis of lysates from C6 cells using Phospho-AKT1-S473 Rabbit pAb (STJ116369) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-AKT1-S473 antibody (STJ116369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

Choose Options

Western blot analysis of lysates from Jurka cells, using Phospho-AKT1-S473 Rabbit pAb (STJ22011) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody (STJ22011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

Choose Options

Anti-Phospho-AKT1-S473 antibody [P8H9] (STJA0019074)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat/AKT8 murine leukemia virus

$269.79 - $1,820.91

Choose Options

Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

Choose Options

Anti-Phospho-AKT1-S473 antibody [SAA0300] (STJA0019165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

Choose Options

Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Phospho-AKT1 antibody western blot MCF-7 cells STJA0036180

Anti-Phospho-AKT1-Ser124 antibody [R04-4X5] (STJA0036180)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Western blot analysis of various cells using Phospho-Akt1 (S129) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser129 antibody (95-144 aa) (STJ90484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-AKT1-Ser129 antibody (STJ99594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Phospho-AKT1 S129 antibody western blot MCF-7 STJA0036168

Anti-Phospho-AKT1-Ser129 antibody [R05-1C4] (STJA0036168)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Western blot analysis of various cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser246 antibody (212-261 aa) (STJ90165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-AKT1-T308 Rabbit pAb (STJ11103747) at 1:1000 dilution. HeLa cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-T308 antibody (STJ11103747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-AKT1-T308 Rabbit pAb (STJ22013) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-AKT1-T308 antibody (STJ22013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

Choose Options

Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

Choose Options

Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 Rabbit pAb (STJ22008) at 1:600 dilution. RD cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AKT1-T450 antibody (STJ22008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb (STJ11102572) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PDGF (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-T450 antibody [S2MR] (STJ11102572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

Choose Options

Anti-Phospho-AKT1-Thr-34 antibody (STJA0005253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rabbit/Rat

$504.39

Choose Options

Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Phospho-AKT1 Thr450 antibody western blot U2OS C6 STJA0035050

Anti-Phospho-AKT1-Thr450 antibody [R07-4A7] (STJA0035050)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

$184.23 - $388.47

Choose Options

Phospho-AKT1 antibody western blot 3T3 lysates STJA0035669

Anti-Phospho-AKT1-Thr450 antibody [R72-1U-9] (STJA0035669)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

Choose Options

Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit polyclonal antibody (STJ11105868) at 1:900 dilution. 293F cells, 293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT2-S474 antibody (STJ11105868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

Choose Options

Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$198.03 - $853.53

Choose Options

Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1：500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30', using APC (Phospho-Ser2054) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-APC-Ser2054 antibody (2020-2069 aa) (STJ90884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb (STJ11102596) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-BAD-S112 antibody [S6MR] (STJ11102596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

Choose Options

Anti-Phospho-Bad-S112 antibody [SRM13686] (STJ11108416)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

Choose Options

Western blot analysis of various cells using Phospho-Bad (S112) Polyclonal Antibody

Anti-Phospho-BAD-Ser112 antibody (78-127 aa) (STJ90188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Phospho-Bad antibody western blot HeLa Calcyculin A STJA0036690

Anti-Phospho-Bad-Ser112 antibody [R07-7Y9] (STJA0036690)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BAD-Ser134 antibody (100-149 aa) (STJ91070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various cells using Phospho-Bad (S136) Polyclonal Antibody

Anti-Phospho-BAD-Ser136 antibody (102-151 aa) (STJ90189)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various cells using Phospho-Bad (S155) Polyclonal Antibody

Anti-Phospho-BAD-Ser155 antibody (119-168 aa) (STJ90190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options