Programmed Cell Death | Research Area | St John's Laboratory

Anti-ELNE antibody [PT0880R] (STJA0039133)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human

$393.30 - $745.20

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Human colon adenocarcinoma stained with MOC-31 antibody using peroxidase-conjugate and DAB chromogen. Note the membranous staining of tumor cells.

Anti-ESA antibody [MOC‐31] (STJ180098)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$222.87 - $725.19

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g FADD antibody (STJ28382). Western blot was performed from the immunoprecipitate using FADD antibody (STJ28382) at a dilution of 1:1000.

Anti-FADD antibody (1-208) (STJ28382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using FADD Polyclonal Antibody diluted at 1：500

Anti-FADD antibody (159-208 aa) (STJ93021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using FADD antibody (STJ11101587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FADD antibody (20-150) [S7MR] (STJ11101587)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot detection of FADD in A431 and Jurkat cell lysates using FADD mouse mAb (1:1000 diluted).Predicted band size:23KDa.Observed band size:23KDa.

Anti-FADD antibody [7C4-F12-F1] (STJ99305)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$205.62 - $496.80

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Anti-FADD antibody [PT1179R] (STJA0040200)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

$207.00 - $745.20

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Western blot analysis of extracts of various cell lines, using Fas antibody (STJ23628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-FAS antibody (200-335) (STJ23628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of human-liver-cancer tissue. 1, FAS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-FAS antibody (257-306 aa) (STJ93041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Dog

$67.62 - $360.18

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Western blot analysis of various cells using CD95 Polyclonal Antibody diluted at 1：500

Anti-FAS antibody (281-330 aa) (STJ92150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of Fas in paraffin-embedded rat kidney using Fas Rabbit monoclonal antibody (STJ11101711) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-FAS antibody (50-150) [S1MR] (STJ11101711)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Western blot analysis of extracts of HT-1080 cells, using Fas Rabbit monoclonal antibody antibody (STJ11104597) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FAS antibody (50-150) [S4597RM] (STJ11104597)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $765.21

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Western blot analysis of HEB cells using FAS Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-FAS antibody (51-100 aa) (STJ96891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot detection of FAS (C-terminus) in Jurkat cell lysates using FAS (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.

Anti-FAS antibody (C-Term) [4C3-G7-B7-H5] (STJ99086)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$205.62 - $496.80

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Anti-Fas antibody [PT0128R] (STJA0039648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$207.00 - $745.20

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Western blot analysis of lysates from 1) HepG2, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5G3) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-FASLG antibody (101-150 aa) (STJ93042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Pig

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer, antibody was diluted at 1:100

Anti-FASLG antibody (121-170 aa) (STJ99000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of mouse heart, using FasLG antibody (STJ23630) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-FasLG antibody (154-170) (STJ23630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse pancreas using Flotillin 1 Rabbit polyclonal antibody (STJ11104576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-FLOT1 antibody (128-427) (STJ11104576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Flotillin 1 antibody (STJ27976). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (STJ27976) at a dilution of 1:1000.

Anti-FLOT1 antibody (155-427) (STJ27976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded human tonsil tissue using Flotillin 1 Rabbit monoclonal antibody (STJ11101835) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-FLOT1 antibody (328-427) [S5MR] (STJ11101835)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-FLOT1 antibody (STJ194764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Mouse heart using FLOT2 Rabbit polyclonal antibody (STJ11104327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FLOT2 antibody (239-428) (STJ11104327)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Western blot analysis of various cells using Flotillin-2 Polyclonal Antibody

Anti-FLOT2 antibody (95-144 aa) (STJ93092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Flotillin 1 antibody [SRM15076] (STJ11109806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using FNTA Rabbit pAb (STJ111424) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-FNTA antibody (280-379) (STJ111424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-FNTA antibody (STJA0008051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Endogenous CDH1 detected with anti-CDH1 at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-FZR1 antibody (601 to 701) (STJ140099)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

$137.31 - $515.43

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Endogenous CDH1 detected at 1:500 dilution lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-FZR1 antibody (750 to 850) (STJ140027)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

$137.31 - $515.43

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Immunohistochemistry analysis of paraffin-embedded human liver cancer using GAS2 antibody (STJ23749) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GAS2 antibody (1-100) (STJ23749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of lysates from Rat liver, using GAS2 Rabbit monoclonal antibody (STJ11105146) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GAS2 antibody (1-100) [S5146RM] (STJ11105146)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GAS2 antibody (STJ190841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Gelsolin antibody [SRM14503] (STJ11109233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

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Human tonsil stained with anti-Granzyme B antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic granular staining of cytotoxic T-cells.

Anti-Granzyme B antibody (73-187aa) [ZM66] (STJ180310)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$222.87 - $758.31

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Anti-Granzyme B antibody (STJ16101330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$901.83 - $1,624.95

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Anti-Granzyme B antibody [11F1] (STJ16101744)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$1,210.95

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Anti-Granzyme B antibody [GrB-7] (STJ16100752)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/ELISA/IHC-P

Reactivity :

Human

$733.47

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Anti-Granzyme B antibody [SM0403] (STJA0010403)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$235.29 - $322.23

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Anti-Granzyme B antibody [SM0404] (STJA0010404)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Rat

$235.29 - $322.23

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Immunofluorescence analysis of un-treated (left) A549 and UV treated (right) A549 cell. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-GSDMD antibody (1-100) (STJA0010253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit monoclonal antibody (STJ11105210) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-GSDMD antibody (276-484) [S5210RM] (STJ11105210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GSDMD antibody (STJ11100237). Western blot was performed from the immunoprecipitate using GSDMD antibody (STJ11100237) at a dilution of 1:500.

Anti-GSDMD antibody (346-484) (STJ11100237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of U2OS cells using GSDMD Rabbit polyclonal antibody (STJ119438) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSDMD antibody (346-484) (STJ119438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit monoclonal antibody (STJ11103428) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-GSDMD antibody (346-484) [S8MR] (STJ11103428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-GSDMD antibody (AA range: 1-100) [PTR1398] (STJA0038893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates, using GSDMD (Full Length+N terminal) Rabbit pAb (STJ11106595) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-GSDMD antibody (Full Length + N-Term) (STJ11106595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of GSDMD in Jurkat lysates using GSDMD antibody.

Anti-GSDMD antibody (Full-length) [R06-5C5] (STJA0013051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$365.01

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Anti-GSDMD antibody [SAA1652] (STJA0020080)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$293.25 - $1,634.61

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