• Western blot analysis of lysates from 293 cells, using FAS Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunofluorescence analysis of HeLa cells, using FAS Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using FAS Polyclonal Antibody
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1, FAS Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of human-kidney tissue. 1, FAS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-kidney tissue. 1, FAS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-liver-cancer tissue. 1, FAS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-liver-cancer tissue. 1, FAS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-FAS antibody (257-306 aa) (STJ93041)

SKU:
STJ93041

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tumor necrosis factor receptor superfamily member 6 (257-306 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IF 1:50-300
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAS
Gene ID: 355
Uniprot ID: TNR6_HUMAN
Immunogen Region: 257-306 aa
Specificity: FAS Polyclonal Antibody detects endogenous levels of FAS protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human FAS at the amino acid range 257-306
Post Translational Modifications (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions. Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 6
Apo-1 Antigen
Apoptosis-Mediating Surface Antigen Fas
Faslg Receptor
Cd Antigen Cd95
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-6803211
Reactome: R-HSA-69416
Reactome: R-HSA-75157
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Isoform 5: Secreted
Isoform 6: Secreted
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 6 antibody
Anti-Apo-1 Antigen antibody
Anti-Apoptosis-Mediating Surface Antigen Fas antibody
Anti-Faslg Receptor antibody
Anti-Cd Antigen Cd95 antibody
Anti-FAS antibody
Anti-APT1 antibody
Anti-FAS1 antibody
Anti-TNFRSF6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance